Identification and evaluation of salt tolerance of Tigernut germplasm resources at seedling stage |
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DOI:10.7606/j.issn.1000-7601.2023.02.01 |
Key Words: Tigernut germplasm seedling stage salt tolerance identify |
Author Name | Affiliation | YU Furong | School of Life Science, Jilin Normal University, Siping, Jilin 136000, China Agricultural Biotechnology Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130124, China | LI Yidan | Agricultural Biotechnology Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130124, China | CHENG Yunqing | School of Life Science, Jilin Normal University, Siping, Jilin 136000, China | YANG Xiangdong | Agricultural Biotechnology Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130124, China | REN Wei | Agricultural Biotechnology Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130124, China |
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Abstract: |
The salt tolerance of thirty\|nine germplasm resources of Tigernut (Cyperus esculeutus L.) from China and abroad were evaluated by measuring 17 physiological indicators using principal component analysis (PCA), cluster analysis, membership function and other methods under the normal and 1.2% NaCl treatment conditions. The results showed that the coefficient of variation of each indicator ranged from 0.128 to 0.455. All germplasms showed highly significant differences (P<0.01) in leaf width, yellowing rate, root length, fresh weight of aboveground and underground parts; and significant differences (P<0.05) in chlorophyll content and tiller number between the NaCl treatment and the normal conditions. According to the result of PCA, 17 variables was transformed into 5 independent comprehensive indicators whose contribution rate reached 80.11%. Dry weight, chlorophyll content, stem diameter, root\|shoot ratio, tiller number and tissue water content could be the main evaluation indicators for salt tolerance. All germplasms were clustered into 3 grades. According to the analysis results of CDC value and D value, three salt\|tolerant germplasms of JYD-35, JYD- 40 and JYD-3 were identified. |
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