| Growth\|promoting bacteria screening from Selenium watermelon rhizosphere and their growth promoting effects |
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| DOI：10.7606/j.issn.1000-7601.2023.02.24 |
| Key Words: plant growth\|promoting rhizobacteria (PGPR) Selenium watermelon screening 16S rDNA identification growth\|promoting effect |
| Author Name | Affiliation | | ZHU Tengteng | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021, China | | HE Xuelian | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021, China | | MIAN Xiaojuan | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021, China | | MA Xian | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021, China | | ZHENG Rui | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021, China | | YUE Sijun | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021, China | | SU Jianyu | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021, China |
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| Abstract: |
| The purpose of this study was to screen and obtain the rhizosphere growth\|promoting bacteria of Selenium watermelon and provide native strain resources and theoretical basis for the development and utilization of Selenium watermelon microbial fertilizer. 50 native strains were screened from the rhizosphere soil of the endemic commercial crop Selenium watermelon in Ningxia by dilution plate method. The ability to solubilize phosphorus, produce iron carrier, produce indole acetic acid (IAA) and fix nitrogen was evaluated. The excellent growth promoting bacteria were identified by 16S rDNA sequence analysis, and nine strains were selected to study their growth\|promoting effects on Selenium watermelon seedlings. The results showed that the ability of 22 strains phosphorus solubilizing was 7.12~64.54 mg·L-1. Using Chrome Azurol S (CAS) plate method, 30 strains were able to produce siderophores. The quantitative results showed that 11 strains were high siderophore\|producing strains. Additionally, the capacities of 18 strains to secrete IAA were 3.70~58.94 mg·L-1. The qualitative results of Ashby’s nitrogen\|free plate showed that 9 strains still grew normally after five generations, which was considered to have the ability of nitrogen fixation. The 20 excellent PGPR strains screened belong to Bacillus, Pseudomonas, Enterobacter, Acinetobacter and Proteus， respectivley. Among them, 6 strains had the above four kinds of growth promoting activities, and 11 strains had the functions of phosphorus solubilizing, siderophore\|producing and IAA secretion. Inoculation of growth promoting bacteria promoted the radicle, hypocotyl, fresh weight and dry weight of Selenium watermelon seedlings. |
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