| Identification of potato serine carboxypeptidase\|like protein StSCPL family and analysis of its response to drought stress |
|
View Fulltext View/Add Comment Download reader |
| |
| DOI：10.7606/j.issn.1000-7601.2023.04.02 |
| Key Words: potato SCPL gene family identification stress response LncRNA |
| Author Name | Affiliation | | JIN Xin | State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China
College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WEI Yuxi | College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | CHEN Zhengliang | College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Zemin | State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China
College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | ZHANG Guodong | Department of Biology, Xinzhou Teachers University, Xinzhou, Shanxi 034099, China | | YANG Jiangwei | State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China
College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | ZHANG Ning | State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China
College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | SI Huaijun | State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China
College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
|
| Hits: 821 |
| Download times: 532 |
| Abstract: |
| The SCPL gene in potato was analyzed and identified at the genomic and transcriptome levels, and the basic characteristics of the SCPL family members were examined. Using drought tolerant cultivar ‘Longshu 10’ (L) and drought sensitive cultivar ‘Atlantic’ (D) as experimental materials, and the key differentially expressed genes of StSCPLs under drought stress were analyzed by high\|throughput sequencing. The results showed that a total of 41 genes were distributed asymmetrically in 11 chromosomes, and most of the genes contained multiple introns. Its encoded proteins containing a signal peptide of cellular endocrine and transport, substrate binding sites, N-glycosylation sites and evolutionarily conserved regions related to catalysis. Under abiotic stress, most StSCPLs were multi\|stress responsive. The potato StSCPLs showed differential expression characteristics in drought differential varieties. At the same time, LncRNA and its target StSCPL-mediated regulatory network showed that StSCPL3 and StSCPL34 were the target genes of lncRNA MSTRG.2301.1 and MSTRG.19548.1, respectively, which were significantly differentially expressed in varieties with different drought tolerance and might be a potential target for potato response to drought stress. |
|
|
|