| Evaluation of drought tolerance and identification of QTLs in single\|segment substitution lines of wild rice at bud stage |
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| DOI：10.7606/j.issn.1000-7601.2023.04.04 |
| Key Words: single chromosome segment substitution line wild rice drought tolerance evaluation QTL identification |
| Author Name | Affiliation | | HUANG Xiuyan | College of Life Sciences，South China Agricultural University, Guangzhou, Guangdong 510642, China | | LI Junbo | College of Agriculture，South China Agricultural University, Guangzhou, Guangdong 510642, China | | LIAO Bing | College of Life Sciences，South China Agricultural University, Guangzhou, Guangdong 510642, China | | FU Xuelin | College of Agriculture，South China Agricultural University, Guangzhou, Guangdong 510642, China | | HE Ping | College of Life Sciences，South China Agricultural University, Guangzhou, Guangdong 510642, China |
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| Abstract: |
| Sixty single segment substitution lines (SSSLs) from O. glumaepatula and O. meridionalis and their recipient parents ‘Huajingxian 74’ (‘HJX74’) were used as test materials under 20% PGE-6000 simulated drought stress in 2020-2021. The relative values of six growth and developmental traits (relative shoot length, relative root length, relative germinal sheath length, relative root number, relative root dry weight, relative shoot dry weight) were used as evaluation indexes. The affiliation function method was used to rank the SSSLs and screen the SSSLs with significantly different traits from ‘HJX74’. QTL identification and additive effect analysis were performed to initially identify the QTLs associated with drought tolerance traits at bud stage. The results showed that the mean affiliation function values of the test SSSLs ranged from 0.14 to 0.71, the mean affiliation function value of ‘HJX74’ was 0.58, and the mean affiliation function values of 12 SSSLs were greater than that of ‘HJX74’. Two drought\|tolerant SSSLs with relative shoot length as the index and four drought\|tolerant SSSLs with relative root number as the index were selected by one\|way ANOVA. Under 20% PGE-6000 simulated drought stress treatment, relative shoot length and relative root number at day 8 of germination were used as indicators to identify drought tolerance QTLs. 2 SSSLs (X24, X8) identified 1 relative shoot length QTL, qRSL1-1, with additive effects of 0.07 and 0.08 and phenotypic contributions of 11.17% and 13.85%, respectively. 4 SSSLs (M77, M120, M133 and M142) identified 3 relative root number QTLs, qRRN10-1, qRRN11-1 and qRRN12-1, with additive effects of 0.11 to 0.24 and phenotypic contributions of 16.64% to 36.34%. The results indicated that these SSSLs carried drought tolerance QTLs at bud stage and can be used as material for drought tolerance gene discovery in rice. |
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