| Response of different genotypes of barley to drought stress at seedling stage and analysis of HvPIPs gene expression characteristics |
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| DOI：10.7606/j.issn.1000-7601.2023.04.05 |
| Key Words: barley seedling stage drought stress HvPIPs |
| Author Name | Affiliation | | HAO Jing | State key Laboratory of Aridland Crop Science, The State Key Laboratory of Crop Genetic Improvement and Germplasm Innovation of Gansu Province, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | YAO Lirong | State key Laboratory of Aridland Crop Science, The State Key Laboratory of Crop Genetic Improvement and Germplasm Innovation of Gansu Province, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Juncheng | State key Laboratory of Aridland Crop Science, The State Key Laboratory of Crop Genetic Improvement and Germplasm Innovation of Gansu Province, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | SI Erjing | State key Laboratory of Aridland Crop Science, The State Key Laboratory of Crop Genetic Improvement and Germplasm Innovation of Gansu Province, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | YANG Ke | State key Laboratory of Aridland Crop Science, The State Key Laboratory of Crop Genetic Improvement and Germplasm Innovation of Gansu Province, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | QIAO Yan | College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | MENG Yaxiong | State key Laboratory of Aridland Crop Science, The State Key Laboratory of Crop Genetic Improvement and Germplasm Innovation of Gansu Province, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | MA Xiaole | State key Laboratory of Aridland Crop Science, The State Key Laboratory of Crop Genetic Improvement and Germplasm Innovation of Gansu Province, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | LI Baochun | State key Laboratory of Aridland Crop Science, The State Key Laboratory of Crop Genetic Improvement and Germplasm Innovation of Gansu Province, Lanzhou, Gansu 730070, China
College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | SHANG Xunwu | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Huajun | State key Laboratory of Aridland Crop Science, The State Key Laboratory of Crop Genetic Improvement and Germplasm Innovation of Gansu Province, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| To study the response of different genotypes of barley to drought stress at seedling stage and explore the germplasm resources of high\|quality drought\|resistant barley, 4 batches of barley with different genotypes of 2 highly resistant varieties ZDM5430 and ZDM5458 and 2 drought\|sensitive varieties 7DCADA and IL-12 were used as materials, and polyethylene glycol (PEG-6000) was used to simulate drought conditions.Differences in drought resistance at seedling stage of different varieties of barley were comprehensively analyzed. Through real\|time quantitative analysis of PIPs in the root systems of different cultivars, the response relationship between aquaporin AQPs genes and drought stress was studied. The results showed that barley growth was slowed down by continuous drought stress, and the seedling length, root length, leaf water content and root water content of all treatments had a downward trend. Of which the leaf water content of the four varieties decreased most significantly during drought stress for 14 days, and the decreases were 29.14%, 52.64%, 21.67% and 72.15%, respectively. The effects of drought stress on dry matter accumulation and root\|shoot ratio at seedling stage of barley were small, and the differences between treatments were not significant. However, after continuous drought, the root\|shoot ratio of ZDM5430 showed an upward trend, which increased by 4.92%, 42.86% and 21.05% compared with CK under stress for 7, 14 and 21 days, respectively. With the extension of stress time, the chlorophyll content and root activity of barley banner also showed a downward trend, and ZDM5430 and ZDM5458 decreased less than those in the control group, indicating that the morphological indexes, chlorophyll content and root vitality of drought\|resistant cultivars were less affected by drought stress. Analyzing the relative expression of PIPs found that under the same treatment conditions, the change trend of HvPIPs in the root system of four different varieties of barley was basically similar. And most of the genes of two subfamilies showed an upward regulation trend after drought stress, and only HvPIP;1, HvPIP1;2, HvPIP2;1 and HvPIP2;5 had a downward trend.The expression of ZDM5430 and ZDM5458 was significantly higher than that of 7DCADA and IL-12. The results showed that this gene was involved in the adaptive regulation of barley to drought stress, and its expression in drought\|tolerant varieties was significantly higher than in drought\|sensitive varieties. |
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