| Physiological tolerance of different herbaceous plants to copper\|nickel combined stress |
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| DOI：10.7606/j.issn.1000-7601.2023.04.18 |
| Key Words: herbaceous plant copper\|nickel combined stress physiological tolerance identification metrics |
| Author Name | Affiliation | | LI Jingfeng | College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, Gansu 730070, China | | GUO Quanen | Soil Fertilizer and Water\|Saving Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China | | LIANG Pengfei | College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, Gansu 730070, China | | HE Haipeng | College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, Gansu 730070, China | | NAN Lili | College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, Gansu 730070, China |
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| Abstract: |
| To explore the physiological growth response and tolerance of herbaceous plants under copper\|nickel combined stress, four main herbs of Lolium perenne ‘Cutter’, Poa pratensis ‘Midnight’, Medicago sativa ‘Qingshui’ and Astragalus sinicus‘Lutana’ in Gansu Province were planted and their biomass and physiological biochemical indexes were determined by setting different copper\|nickel compound stress concentrations of 0, 100, 200, 400, 800 and 1 600 mg·kg-1. Linear stepwise regression and membership function analysis were used to comprehensively evaluate the tolerance of four herbs under copper\|nickel combined stress. The results showed that the plant height, aboveground and underground biomass, chlorophyll a, chlorophyll b, total pigment content of chloroplasts, relative water content and root activity of the four plants were significantly reduced by copper\|nickel compound stress (P<0.05). Compared with CK, the decrease ranges were 86.83%~90.54%, 49.40%~61.50%, 44.44%~82.25%, 24.47%~44.26%, 45.91%~70.22%, 25.04%~36.59%, 17.59%~20.98% and 14.71%~69.32%, respectively. The root\|crown ratio, soluble sugar and soluble protein content, SOD, POD and CAT activities increased first and then decreased, and the peak increase range was 1.42%~35.88%, 264.91%~743.65%, 65.93%~327.81%, 23.23%~86.38%, 131.62%~176.93% and 72.42%~85.87%, respectively. The contents of malondialdehyde, free proline and carotenoids increased significantly (P<0.05) ranged from 40.62%~62.90%, 235.34%~1060.04%, 576.17%~1282.58%, respectively. The content of Cu2+ and Ni2+ in roots, stems and leaves increased, and the contents of Cu2+ and Ni2+ in different organs were different. Regression analysis screened subsurface biomass, soluble sugars, soluble proteins, proline, Cu2+ content and SOD activity as key indicators for selecting copper\|nickel\|resistant plants. The copper\|nickel tolerance of four herbaceous plants was obtained by the comprehensive evaluation of the membership function: Medicago sativa (0.565) > Astragalus sinicus (0.538) > Poa pratensis (0.449) > Lolium perenne (0.448). This study can be used as a reference for the screening of copper\|nickel resistant plants and soil bioremediation applications. |
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