| Degradation effects of a strain of purple violet spore on polyethylene film |
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| DOI：10.7606/j.issn.1000-7601.2023.04.23 |
| Key Words: purpureocillium lilacinum polyethylene film degradation ability film color |
| Author Name | Affiliation | | WANG Lei | Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai 810016, China
Xining Experimental Station for Scientific Observation of Crop Pests, Ministry of Agriculture and Rural Affairs, Xining, Qinghai 810016, China
Key Laboratory for Comprehensive Control of Agricultural Pests in Qinghai Province, Xining, Qinghai 810016, China | | HUI Xiangjuan | Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai 810016, China
Xining Experimental Station for Scientific Observation of Crop Pests, Ministry of Agriculture and Rural Affairs, Xining, Qinghai 810016, China
Key Laboratory for Comprehensive Control of Agricultural Pests in Qinghai Province, Xining, Qinghai 810016, China | | ZHI Hui | Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai 810016, China
Xining Experimental Station for Scientific Observation of Crop Pests, Ministry of Agriculture and Rural Affairs, Xining, Qinghai 810016, China
Key Laboratory for Comprehensive Control of Agricultural Pests in Qinghai Province, Xining, Qinghai 810016, China | | SHANGGUAN Zhouping | Key Laboratory for Comprehensive Control of Agricultural Pests in Qinghai Province, Xining, Qinghai 810016, China | | LI Wei | Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai 810016, China
Xining Experimental Station for Scientific Observation of Crop Pests, Ministry of Agriculture and Rural Affairs, Xining, Qinghai 810016, China
Key Laboratory for Comprehensive Control of Agricultural Pests in Qinghai Province, Xining, Qinghai 810016, China |
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| Abstract: |
| To obtain strain resources that could efficiently degrade polyethylene mulch, a strain M2 with the potential to degrade polyethylene provided by Plant Protection Institute of Academy of Agricultural and Forestry Sciences, Qinghai University was used as the research object to explore its ability to degrade different colors (white transparent, black) of polyethylene film. The strain M2 was identified as Purpureocillium lilacinum by morphological observation and ITS sequencing. Under the condition of 30℃, 150 r·min-1 shaking culture, 30 d and 60 d sampling once respectively. The degradation effects of the strain on different color polyethylene film were analyzed by high resolution field emission scanning electron microscope, water contact instrument and Fourier transform infrared spectrometer. Scanning electron microscopy showed that after 30 days of degradation, both films showed slight fragmentation. After 60 days of degradation, both films showed obvious holes, the surface roughness increased significantly, and the black film was degraded more obviously. By measuring the weight loss rate of the film, the degradation rate of M2 on white transparent film reached 7.27%, and the degradation rate of black film reached 8.45%, indicating that the degradation ability of M2 to black film was greater than that of white transparent film. After 30 days of degradation, the hydrophobicity of black film was significantly lower than that of white transparent film by 5.51%. After 60 days of degradation, the hydrophobicity of black film was significantly lower than that of white transparent film by 21.35%. In addition, the surface hydrophobicity of both mulches decreased to varying degrees, and the hydrophobicity of black film was significantly reduced. Fourier transform infrared spectrometer analysis showed that M2 introduced ether bonds into the stable chemical structure of white transparent film, while black film was introduced by hydroxyl groups and ether bonds. The absorption peak intensity of black film was greater than that of white film. It was preliminarily determined that strain M2 had good degradation effects on black polyethylene film. |
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