| Agricultural College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China |
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| DOI：10.7606/j.issn.1000-7601.2023.05.20 |
| Key Words: medium gluten wheat weak gluten wheat postponing nitrogen fertilizer application starch synthesis gelatinization characteristics |
| Author Name | Affiliation | | WU Dongming | Agricultural College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | HAN Yanan | Agricultural College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | MA Hongliang | Agricultural College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | QI Pengfei | Agricultural College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | WEI Yuming | Agricultural College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | FAN Gaoqiong | Agricultural College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | LIU Qiong | Agricultural College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | ZHENG Ting | Agricultural College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China |
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| Abstract: |
| Two medium\|gluten varieties and two weak\|gluten varieties were evaluated at three periods of N fertilizer application, 100% basal，60% basal and 40% topdressing at jointing stage, and 60% basal and 40% topdressing at booting stage, under N level of 150 kg·hm-2 at two sites over two growing seasons. The results showed that, (1) The contents of total starch and amylose and the ratio of amylose to amylopectin in grains, whose percentages of variability explained by postponing N fertilizer application accounted for 23.92%~39.00%, 37.65%~45.08% and 24.29%~29.04%, respectively, for decreasing by 0.20~1.75 percentage points, 0.10~1.85 percentage points and 4.88%~10.42%, respectively, while no change was observed in the amylopectin content. (2) The period of nitrogen application explained 0.01%~13.5% variation of gelatinization characteristics. The pasting indexes of the four varieties were basically improved after postponing N fertilizer application. Significant differences were identified in changes of peak viscosity, trough viscosity, final viscosity and recovery value. (3) The activities of AGPase, SSS and GBSS in grains exhibited decreasing trends during filling stage after postponing N fertilizer application, and significant differences were widely detected during 20~35 days after anthesis, especially for the medium\|gluten cultivars. (4) The expression of AGP-L, Sbe1D，and Wx-DI significantly decreased during 20~35 days after anthesis. Collectively, the formation and accumulation of starch was inhibited to some extent after postponing N fertilizer application. It was recommended that 100% basal or topdressing at jointing stage was suitable for weak\|gluten varieties, topdressing at booting stage was suitable for medium\|gluten varieties. |
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