| Expression analysis of potato StTCP7 gene under drought and salt stress |
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| DOI：10.7606/j.issn.1000-7601.2023.06.04 |
| Key Words: potato StTCP7 gene cloning drought stress salt stress expression analysis |
| Author Name | Affiliation | | ZHAO Yongxian | College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China
State Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | WANG Kaitong | State Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | ZHANG Ning | College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China
State Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | SI Huaijun | College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China
State Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China |
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| Abstract: |
| To explore the expression pattern of TCP7 gene in potato (Solanum tuberosum L.) under drought and salt stress, the pot seedlings of drought\|tolerant variety ‘Desiree’ and the drought\|sensitive variety ‘Atlantic’ used as the test material treating with the natural drought and 200 mmol·L-1 NaCl solution. The StTCP7 gene with CDS region length 741 bp, and promoter region containing action elements in response to drought induction was cloned by PCR, which encoded a protein containing 246 amino acids. the protein sequence of StTCP7 was the most similar to TCP7 (Solanum stenotomum) with homology of 98.2%, and this protein contains one conserved TCP domain and belongs to the alkaline protein, which is located on the nucleus. Using qRT-PCR, the analysis of StTCP7 gene expression in ‘Desiree’ and ‘Atlantic’ under drought and salt stress revealed that there were organ and inter\|breed differences. Expression in ‘Desiree’ was expressed as root (1.02)> stem (0.33)> leaf (0.29), and in ‘Atlantic’ as leaf (4.62)> stem (3.72)> root (1.00). Under the drought stress, the expression level of StTCP7 gene in ‘Desiree’ roots and leaves increased first and then decreased with the degree of drought stress, reaching the peak at W3 (soil moisture was 35%~45%), and its expression level was 1.27 and 1.87 times that of the control group respectively. The expression level of the gene in ‘Atlantic’ roots increased first and then decreased, reaching the peak at W3 (35%~45%), and its expression level was 1.39 times that of the control group. The expression level in leaves showed a continuous upward trend, reaching the peak at W4 (15%~25%), and its expression level was 2.52 times that of the control group. As for the salt treatment, the expression level of StTCP7 gene in ‘Desiree’ roots increased first and then decreased with increasing salt stress time, and the expression level reached the highest at 6 h, which was 2.16 times that of the control group. The expression level in leaves increased first and then decreased, reaching the peak at 3 h, and its expression level was 4.54 times that of the control group. The expression level of the gene in ‘Atlantic’ roots increased first and then decreased, reaching the highest at 3 h, which was 2.12 times that of the control group. The expression level in leaves showed an upward trend, reaching the peak at 48 h, and its expression level was 4.04 times that of the control group. To sum up, StTCP7 gene responded to drought and salt stress, and its expression in different organs of roots and leaves was different, which would provide a basis for functional research of potato StTCP7 gene in abiotic stress response. |
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