| Identification and expression analysis of APX gene family in celery (Apium graveolens L.) |
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| DOI：10.7606/j.issn.1000-7601.2023.06.06 |
| Key Words: Apium graveolens L. APX system evolution cis\|element high temperature response |
| Author Name | Affiliation | | DU Jiageng | College of Horticulture，Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | ZHOU Jin | College of Horticulture，Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | LI Xiaoyan | College of Horticulture，Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | ZHANG Ying | College of Horticulture，Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | WU Limei | College of Horticulture，Sichuan Agricultural University, Chengdu, Sichuan 611130, China | | LI Mengyao | College of Horticulture，Sichuan Agricultural University, Chengdu, Sichuan 611130, China |
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| Abstract: |
| Ascorbate peroxidase (APX) is one of the important antioxidant enzymes in plant reactive oxygen species metabolism and plays an important role in plant resistance to oxidative stress. In this study, bioinformatics was used to identify and analyze APX gene family members in celery genome, and the expression of AgAPXs under high temperature stress was verified and analyzed by quantitative real\|time PCR (qRT-PCR), which provided a basis for participation of celery APX gene in the regulation mechanism of high temperature stress. Results showed that a total of 9 APX genes were systematically identified from celery, which were randomly distributed on 5 chromosomes, and gene fragment duplication occurred. Most genes were located in the cytoplasm. Phylogenetic analysis showed that the AgAPX gene family could be divided into three subtypes, and members of each subtype shared similar gene structures and motifs. Promoter cis element analysis showed that most AgAPX genes contain a variety of cis elements related to growth and development, plant hormones and stress. Under high temperature stress, the APX activity of celery increased. qRT-PCR analysis showed that AgAPXs were differentially expressed under high temperature treatment at different times, were consistent with the transcriptome expression abundance. The expression of AgAPX2, AgAPX3, AgAPX4, AgAPX5 and AgAPX7 was significantly correlated with the activity of APX, which could be speculated that this gene was involved in the regulation of celery tolerance to high temperature. The results preliminarily identified and provided information on the members of celery APX gene family, which provided an important research basis for further research on the function of celery APX gene in the future. |
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