| Drought response during flowering stage of new type Brassica napus L. derived from interspecific hybridization between B. juncea × B. napus |
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| DOI：10.7606/j.issn.1000-7601.2023.06.09 |
| Key Words: Brassica napus L. interspecific hybrid of B. juncea×B. napus flowering drought physiological response |
| Author Name | Affiliation | | CHENG Tiantian | Xizang Agriculture and Animal Husbandry College, Linzhi， Xizang 860000, China | | CHEN Jia | Xizang Agriculture and Animal Husbandry College, Linzhi， Xizang 860000, China | | LAI Ming | Xizang Agriculture and Animal Husbandry College, Linzhi， Xizang 860000, China | | ZHANG Jun | Xizang Agriculture and Animal Husbandry College, Linzhi， Xizang 860000, China | | LIU Hongyu | Xizang Agriculture and Animal Husbandry College, Linzhi， Xizang 860000, China | | MENG Zuqing | Xizang Agriculture and Animal Husbandry College, Linzhi， Xizang 860000, China | | SONG Fengping | Xizang Agriculture and Animal Husbandry College, Linzhi， Xizang 860000, China |
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| Abstract: |
| The contents of malondialdehyde, proline and antioxidant enzyme activities of new Brassica napus L. under drought were studied by crossing two varieties (‘290’ and ‘299’) of brassica napus with different drought resistance, under the three drought treatments of T1 (13%~17% soil moisture in 10 cm layer), T2 (7%~9% soil moisture in 10 cm layer), and CK (48%~49% soil moisture in 10 cm layer). The results showed as follows: (1) The accumulation of malondialdehyde in leaves and buds of rapeseed was induced by drought treatment at flowering stage. The malondialdehyde content in leaves continued to increase with the severity of drought. Drought\|resistant strain ‘290’ and non\|drought\|resistant strain ‘299’ increased by 0.28% and 63.83%, respectively, compared with the control under T1 drought. Under T2 drought, malondialdehyde content in leaves increased by 29.12% and 64.18% compared with control, respectively. The value of chlorophyll a/b decreased continuously, and the content of chlorophyll a/b in the leaves of strains ‘290’ and ‘299’ under T1 drought decreased by 7.43% and 22.70% compared with the control, respectively. Under T2 drought, the content of chlorophyll a/b in the leaves of strains ‘290’ and ‘299’ decreased by 20.59% and 26.61%, respectively, compared with the control. The increase of malondialdehyde content in flower bud was lower than that in control under severe drought. The results showed that under severe drought stress, the cell membrane of flower bud was less damaged by oxidation than that of leaf, and the damage of non\|drought\|resistant strain was more obvious. (2) Drought treatment can induce the increase of proline content and antioxidant enzyme activity in leaves and buds of rapeseed, and the contents of proline and antioxidant enzyme in leaves of mild drought T1 were significantly increased compared with the control. The accumulation of proline in leaves of strains ‘290’ and ‘299’ was increased by 1583.45% and 838.32% compared with the control, respectively. The catalase (CAT) activity in leaves was increased by 185.71% and 173.53%, respectively. The peroxidase (POD) in leaves was increased by 140.52% and 42.80% compared with control. The activity of superoxide dismutase (SOD) in leaves was increased by 93.62% and 68.59%, respectively. With the aggravation of drought stress, the increase of proline in leaves under severe drought T2 decreased compared with that under mild drought T1, and the accumulation of proline in leaves of strain ‘290’ and ‘299’ increased by 992.67% and 635.37%, respectively. CAT activity in leaves was increased by 278.94% and 231.09%, respectively. POD activity in leaves was increased by 85.00% and 47.06% compared with control. The SOD activity of strain ‘290’ in leaves increased by 41.84%, and that of strain ‘299’ decreased by 21.79%, while the activity of SOD in flower buds increased continuously. The drought-resistant strains treated with T1 showed more obvious performance. The results showed that under mild drought, rape mainly activated the defense mechanism of leaves; while under severe drought, stress activated the defense mechanism of buds to prevent the dehydration of buds and ensure the maximum fruiting. (3) After drought treatment, the total number of effective siliqua per plant decreased significantly. After T1 drought treatment, the total number of effective siliqua per plant decreased by 18% and 47%, respectively. After T2 drought treatment, the total number of effective siliqua per plant of strains ‘290’ and ‘299’ decreased by about 18% and 54%, respectively, while the number of nuts per siliqua and 1000\|grain weight suffered relatively little. The results showed that dry matter was preferentially allocated to seeds in the growth stage of drought recovery, thus ensuring siliqua bearing. |
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