| Identification of the pathogen causing root rot of Lycium barbarum and screening of antagonists |
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| DOI：10.7606/j.issn.1000-7601.2023.06.26 |
| Key Words: Lycium barbarum root rot pathogenic bacteria antagonistic bacteria |
| Author Name | Affiliation | | WANG Guoqin | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021， China | | DU Xueqian | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021， China | | MA Cong | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021， China | | ZHU Tengteng | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021， China | | ZHENG Rui | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021， China | | YUE Sijun | College of Life Sciences, Ningxia University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, Ningxia 750021， China |
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| Abstract: |
| To implement effective biological control against root rot disease, the pathogenic bacteria and their antagonistic bacteria were isolated and identified. The pathogens on decaying roots were isolated by tissue isolation method, and the types of fungi were identified by morphological and molecular biological characteristics. Antagonistic bacteria were screened from healthy rhizosphere soil by dilution coating method, filter paper method, and co\|culture method. The strains with good antagonistic effect were identified by morphological observation, physiological and biochemical identification, and 16S rDNA sequence analysis. The results showed that Fusarium solani and Fusarium oxysporum were isolated from the root rot of Lycium barbarum. Twelve strains were isolated from the rhizosphere soil of healthy Lycium barbarum with antagonistic effects against two Fusarium strains. The inhibitory rates of J7-3, J7-8, J7-9 and J10-8 against F. solani were all above 80%, and the inhibitory rates of J7-3 and J10-8 against F. oxysporum were all above 70%. Strains J7-3 and J10-8, identified as Bacillus amyloliquefaciens and Bacillus subtilis, were well against root rot pathogens. Among them, J10-8 had the best antagonistic effect and bacteriolytic effect and can be used as a material resource for the development of microbial agents. |
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