| Evaluation of salt tolerance of 80 introduced alfalfa varieties during germination |
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| DOI：10.7606/j.issn.1000-7601.2024.02.03 |
| Key Words: alfalfa introduced variety germination period salt tolerance evaluation |
| Author Name | Affiliation | | WANG Ning | School of Life Science, Jilin Normal University, Siping, Jilin 136000, China
Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun, Jilin 130124, China | | WAN Chang | Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun, Jilin 130124, China | | GAO Shan | Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun, Jilin 130124, China | | YAN Chengming | Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun, Jilin 130124, China | | LI Zhonghe | Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun, Jilin 130124, China | | WANG Junqiao | Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun, Jilin 130124, China | | REN Wei | Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun, Jilin 130124, China | | WANG Zhifeng | Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun, Jilin 130124, China |
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| Abstract: |
| In this study, the effects of salt stress on root length, bud length, germination index, germination rate, germination energy, vitality index and root\|bud ratio of 80 introduced alfalfa varieties treated with 1.2% NaCl were studied through laboratory germination test. A comprehensive evaluation of the salt tolerance of alfalfa during its germination period was conducted using a combination of principal component analysis, membership function, and cluster analysis. The results showed that salt stress significantly affected the germination energy, germination rate, and germination index of alfalfa (P<0.01), and the coefficient of variation between different varieties ranged from 0.008 to 0.723. The cumulative contribution rate of the two principal components was 73.922%. The germination energy and root\|bud ratio were used as the main salt tolerance indicators for salt tolerance screening during the germination period of alfalfa. The clustering analysis results divided 80 alfalfa varieties into 5 levels, including 9 varieties with strongest salt tolerance, 14 varieties with strong salt tolerance, 43 varieties with moderate salt tolerance, 13 varieties with weak salt tolerance, and 1 variety with the weakest salt tolerance. On this basis, varieties of alfalfa with strong salt tolerance during the germination period were selected as follows: WL168HQ, Tourists, Tianma, Dite, Juneng 801, and Juneng 995. |
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