| Effects of compound anti\|salt agent on seed germination and growth of wheat under NaCl stress |
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| DOI：10.7606/j.issn.1000-7601.2024.02.22 |
| Key Words: compound anti\|salt agent wheat salt stress seed germination resistance physiology |
| Author Name | Affiliation | | LIU Xiaochen | Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China
Nanjing Forestry University, Nanjing, Jiangsu 210037, China | | WANG Shufeng | Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China | | SHOU Songtao | Center for Rural Vitalization of Fuyang, Hangzhou, Zhejiang 311400, China | | ZHANG Jianfeng | Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China |
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| Abstract: |
| Seven substances, including KNO3、CaCl2、DA-6、KH2PO4、FeSO4·7H2O、MgSO4·7H2O, and sucrose were used to formulate compound anti\|salt agent. Using ‘Jimai 22’ as test material, the water cultivaton method was employed to investigate the effects of different concentrations of anti\|salt agent (10%, 20% and 30%) on seed germination and subsequent seedling growth under different salt (NaCl) stress (0.2%, 0.4% and 0.6%), and consequently the suitable formulation of anti\|salt agent for wheat was proposed. The results showed that: (1) 30%, 20% and 10% anti\|salt agent had the best effect in promoting wheat seed germination under 0.2%, 0.4% and 0.6% salt stress, and the germination rate increased by 6.4%, 12.0% and 26.5%, respectively. (2) Adding anti\|salt agents at a salt concentration of 0.6%, root dry mass significantly increased by 29.7% to 35.9%. Photosynthetic rate was increased after the addition of anti\|salt agent by 67.5%~90.0% (salt concentration 0.2%), 13.3%~51.5% (salt concentration 0.4%) and 0.4%~25.5% (salt concentration 0.6%), respectively. The 30% anti\|salt agent significantly enhanced photosynthesis at a salt concentration of 0.2%, with an increase of 90.0%. The photosynthetic pigment content of wheat was increased, with Chla content increased by 22.5%~39.0% (salt concentration 0.2%), 41.7% to 43.2% (salt concentration 0.4%); and Chlb content increased by 22.1%~31.0% (salt concentration 0.2%), 14.7%~22.5% (salt concentration 0.4%); and carotenoid content increased by 12.2% to 28.6% (salt concentration 0.2%) and 24.4% to 32.3% (salt concentration 0.4%), respectively. In addition, other physiological indicators also had corresponding improvement. (3) Based on the principal component analysis, the results showed that wheat treated with 30% anti\|salt agent had the highest score, so 30% anti\|salt agent was the most suitable concentration ratio. |
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