| Mutation analysis of phenotypic characteristics of Astragalus membranaceus germplasm resources induced by space mutagenesis |
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| DOI：10.7606/j.issn.1000-7601.2024.03.01 |
| Key Words: Astragalus membranaceus space mutagenesis phenotypic characteristics variation analysis germplasm screening |
| Author Name | Affiliation | | LI Hongling | College of Agronomy，College of Life Sciences and Technology，Gansu Provincial State Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | CHEN Yuan | College of Agronomy，College of Life Sciences and Technology，Gansu Provincial State Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | GUO Fengxia | College of Agronomy，College of Life Sciences and Technology，Gansu Provincial State Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | XU Hongliang | Tianjin Key Lab of Modern Chinese Medicine Resource Research Enterprise, Tianjin Tianshili Modern Chinese Medicine Resources Co., Ltd, Tianjin 300410, China | | LI Ruixia | College of Agronomy，College of Life Sciences and Technology，Gansu Provincial State Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| The 33D and 22H populations established by Astragalus membranaceus with seeds in space and the SP2-generation adult strains without seeds in space were selected as the research objects. The characteristic variation and diversity of 19 phenotypic characters were analyzed to screen the germplasm resources of Astragalus membranaceus with excellent characters.The results showed that the variation amplitude and genetic diversity of 19 traits in mutagenic progeny population were different to various degrees. The variation coefficient CV of four quality traits in the 33D, 22H and CK populations were 0.35 to 0.78, 0.38 to 0.84, and 0.41 to 0.64, respectively, and the variation range was 22H>33D>CK. The range of genetic diversity index H′ was 1.67 to 2.13, 1.66 to 2.02, and 1.44 to 2.21, respectively, and the variation range was CK>33D>22H. CV of the seven quantitative traits ranged from 0.17~0.62, 0.17~0.67 and 0.20~0.83, respectively. H′ of genetic diversity index ranged from 1.47 to 2.01, 1.72 to 2.04， and 1.59 to 2.11, respectively, indicating the range of variation was CK>22H>33D. The variation coefficients of the eight yield components ranged from 0.09 to 0.53, 0.15 to 0.55, and 0.09 to 0.97, respectively, and the variation range was CK>22H>33D, the H′ of genetic diversity index ranged from 1.81 to 2.09, 1.73 to 2.06 and 1.59 to 2.03, respectively, and the range of variation was 33D>22H>CK. By principal component factor analysis, 12 quantitative traits of mutant progeny plants were simplified into 4 principal factors. The cumulative contribution rate of quantitative traits in 33D and 22H populations was higher than those in CK, reaching 68.669% and 70.414%, respectively. The secondary branch number, single stem mass, effective root length, lateral root number, root length and drying rate were selected as the representative indexes for the evaluation of Astragalus membranaceus. After mutagenesis, membership function value of above\|ground traits in SP2-generation was less than CK, and membership function value of yield constituent traits was greater than CK. By membership function and cluster analysis, the two populations of SP2-generation after mutagenesis were classified into three different types of plants\|types I, II and III. Group I was the good type, Group II was the dominant type, and Group III was the reserve type. A comprehensive evaluation found 39 germplasm with excellent traits, 16 germplasm with excellent yield composition traits, and 3 high\|yielding germplasm with dwarf type. |
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