| Amplification of glutathione reductase gene and its response to continuous cropping stress in soybean |
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| DOI：10.7606/j.issn.1000-7601.2024.03.07 |
| Key Words: soybean glutathione reductase continuous cropping stress gene amplification gene expression |
| Author Name | Affiliation | | HAN Bei | College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LI Chen | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | PANG Yuanyuan | College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | FANG Shumei | College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LIANG Xilong | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China |
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| Abstract: |
| The CDS of four GR genes were obtained in the soybean database Phytozome. The GR genes were cloned by RT-PCR using RNA from leaf and root as templates, respectively. The sizes of [STBX]Glyma10g03740[STBZ] and [STBX]Glyma02g16010[STBZ] were both 1 638 bp, indicating similar gene structures. The gene structures of [STBX]Glyma16g27210[STBZ] and [STBX]Glyma02g08180[STBZ] were similar with 1 506 bp. [JP2]Phylogenetic analysis showed that Glyma10g03740/Glyma02g16010 and Glyma16g27210/Glyma02g08180 were clustered in different branches, while they were all closely related to Vigna unguiculata, Phaseolus acutifolius and Phaseolus vulgaris, respectively. The four GRs had the same domains with FAD/NAD binding_ dom and Pyr_ Nucl dis_ OxRdcase_ Dimer. The tertiary structure showed that the conformations of Glyma16g27210 [JP2]and Glyma02g08180 were similar, while those of Glyma10g03740 and Glyma02g16010 exhibited similarity as well. All four GRs existed in homodimers, with identical interacting proteins, including two thioredoxin reductases and eight thioredoxins. The RT-qPCR method was used to analyze the response of GRs to continuous cropping stress. The results showed that under continuous cropping stress, the GRs expression of sensitive variety HF55 was initiated earlier in roots, within 15 days after emergence, but it was activated later in leaves. The expression still showed an upward trend at 45 days after emergence. In the resistant variety KX8, the expression of GRs in roots and leaves reached their highest levels between 15 days and 45 days after emergence. At 30 days, the total expression of GRs in roots and leaves increased by 19.03 times and 2.50 times, respectively. |
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