| Sequencing and analysis of transcriptome on regulating gene expression of Sphallerocarpus gracilis in Qilian Mountains under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2024.03.08 |
| Key Words: Sphallerocarpus gracilis drought stress transcriptomics sequencing differentially expressed genes |
| Author Name | Affiliation | | ZHANG Chunmei | College of Agriculture and Ecological Engineering, Hexi University, Zhangye, Gansu 734000, China
Key Laboratory of Hexi Corridor Resources Utilization of GanSu, Zhangye, Gansu 734000, China | | QI Shiming | College of Agriculture and Ecological Engineering, Hexi University, Zhangye, Gansu 734000, China
Key Laboratory of Hexi Corridor Resources Utilization of GanSu, Zhangye, Gansu 734000, China | | YAN Fang | Key Laboratory of Hexi Corridor Resources Utilization of GanSu, Zhangye, Gansu 734000, China
Ecological & Oasis Agricultural Research Institute of Hexi University, Zhangye, Gansu 734000, China | | ZHAO Gang | College of Agriculture and Ecological Engineering, Hexi University, Zhangye, Gansu 734000, China
Key Laboratory of Hexi Corridor Resources Utilization of GanSu, Zhangye, Gansu 734000, China | | SONG Hai | Key Laboratory of Hexi Corridor Resources Utilization of GanSu, Zhangye, Gansu 734000, China | | ZHANG Xifeng | College of Agriculture and Ecological Engineering, Hexi University, Zhangye, Gansu 734000, China
Key Laboratory of Hexi Corridor Resources Utilization of GanSu, Zhangye, Gansu 734000, China | | CHEN Ye | College of Agriculture and Ecological Engineering, Hexi University, Zhangye, Gansu 734000, China
Key Laboratory of Hexi Corridor Resources Utilization of GanSu, Zhangye, Gansu 734000, China |
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| Abstract: |
| This study used the roots and leaves of 2-month-old S.gracilis from moderate drought stressed group and control group (the relative water content in soil was 55%~60% and 70%~80%, respectively) as the test material. The transcriptome sequencing analysis was carried out by using BGISEQ-500. After obtaining transcriptome data, gene function annotation, and differentially expressed genes (DEGs) screening of S.gracilis leaf and root were performed. The results showed: (1) A total of 34230 (50.20%), 34170 (50.11%), 31727 (46.53%), 27701 (40.62%), 27092 (39.73%), and 22793 (33.42%) were annotated by non\|redundant protein sequence database (NR), eggNOG, gene ontology (GO), Pfam databases, SwissProt and Kyoto Encyclopedia of genes and genomes (KEGG)respectively. (2) There were 10674 and 13402 DEGs in roots and leaves, respectively. At the same time, GO enrichment analysis showed that DEGs of root and leaf were distributed the same in GO functional annotations, mainly focusing on biological process, regulation of transcription, DNA-templated, protein phosphorylation, oxidation\|reduction process and defense response. Moreover, the KEGG pathway analysis showed that DEGs in roots were significantly enriched in phenylpropanoid biosynthesis, galactose metabolism, cysteine and methionine metabolism, starch and sucrose metabolism, plant\|pathogen interaction and plant hormone signal transduction. The DEGs in leaves were mainly concentrated in galactose metabolism, starch and sucrose metabolism, phenylpropanoid biosynthesis, pentose and glucuronate interconversions and plant hormone signal transduction, indicating that drought stress mainly affected starch and sucrose metabolism, galactose metabolism, phenylpropanoid biosynthesis pathways and plant hormone signal transduction in the roots and leaves of S.gracilis as well as these biological pathways played an important role in the response under drought stress. The high\|throughput transcriptome sequencing revealed the regulatory characteristics of moderate drought stress on gene expression in different organs of S.gracilis. In short, drought stress affected the expression of differential genes in different organs. The study can provide a theoretical basis for analyzing the biological pathway of tolerance of S.gracilis, biosynthesis and molecular mechanism of effective components under drought stress. |
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