| Differential metabolites analysis on two varieties of fermented cigar leaves in semi\|arid region |
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| DOI：10.7606/j.issn.1000-7601.2024.04.03 |
| Key Words: cigar varieties tobacco non\|targeted metabolomics differential metabolites semi\|arid region |
| Author Name | Affiliation | | LU Anzhi | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China | | ZHANG Leyi | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China | | WANG Pingping | Shaanxi Institute of Tobacco Science, Xi’an, Shaanxi 710000, China | | CHEN Xinying | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China | | DU Shanshan | Hanzhong Company, Shaanxi Tobacco Company, Hanzhong, Shaanxi 723000, China | | LI Xianfeng | Hanzhong Company, Shaanxi Tobacco Company, Hanzhong, Shaanxi 723000, China | | WANG Hui | Hanzhong Company, Shaanxi Tobacco Company, Hanzhong, Shaanxi 723000, China | | ZHANG Qiang | Shaanxi Institute of Tobacco Science, Xi’an, Shaanxi 710000, China | | AI Suilong | Shaanxi Institute of Tobacco Science, Xi’an, Shaanxi 710000, China | | ZHANG Lixin | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| By comparing the metabolites between two varieties of fermented cigar leaves ‘Haiyan 103’ and ‘Qingxue 204’, the metabolome differences between two varieties of cigar leaves were explored. In the study, non\|targeted metabolomic analysis of the chemical components of two fermented cigar leaves were analyzed by Ultra High\|Performance Liquid Chromatography\|Quadrupole\|Time of Flight Mass Spectrometric (UHPLC-QTOF-MS). The results indicated that, the metabolomes relative content of ‘Haiyan 103’ and ‘Qingxue 204’ were significantly different. A total of 179 differential metabolites were identified in two varieties of fermented cigar leaves, of which 114 metabolites were significantly up\|regulated in ‘Haiyan 103’ compared to ‘Qingxue 204’, while 65 were significantly down\|regulated. Forty major differential metabolites were qualitatively and quantitatively identified, including 10 organic acids, 7 amino acids, 4 phenolic acids, 4 nucleotides and analogues, 3 carbohydrates, 3 flavonoids, 3 lignans and coumarins, and 6 others. Based on KEGG pathway analysis, 12 significantly enriched pathways were identified. |
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