| Effects of freezing stress on leaf structure and physiological indices of Brassica napus L. with different cold resistance |
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| DOI：10.7606/j.issn.1000-7601.2024.05.13 |
| Key Words: winter Brassica napus cold resistance leaf anatomical structure physiology characteristics |
| Author Name | Affiliation | | SHI Yangyang | College of Agronomy， Gansu Agricultural University, Lanzhou, Gansu 730070, China | | PU Yuanyuan | College of Agronomy， Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WU Junyan | College of Agronomy， Gansu Agricultural University, Lanzhou, Gansu 730070, China | | SUN Wancang | College of Agronomy， Gansu Agricultural University, Lanzhou, Gansu 730070, China | | LIU Lijun | Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Lanzhou, Gansu 730070, China | | MA Li | Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Lanzhou, Gansu 730070, China | | WANG Wangtian | College of Agronomy， Gansu Agricultural University, Lanzhou, Gansu 730070, China | | ZHAO Yuhong | Gansu Yasheng Agricultural Research Institute Co. Ltd, Lanzhou, Gansu 730030, China | | YANG Gang | College of Agronomy， Gansu Agricultural University, Lanzhou, Gansu 730070, China | | FAN Tingting | College of Agronomy， Gansu Agricultural University, Lanzhou, Gansu 730070, China | | BIAN Liang | College of Agronomy， Gansu Agricultural University, Lanzhou, Gansu 730070, China | | LU Xiaoming | College of Agronomy， Gansu Agricultural University, Lanzhou, Gansu 730070, China | | MA Rui | College of Agronomy， Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| In this study, different cold\|tolerant Brassica napus L. growing in northern China were treated with freezing to compare their physiological differences. The strong cold\|tolerant line 2016TS(G)10 and the weak cold\|tolerant variety Tianyou 2288 as the materials were subjected to freezing at -2℃. The plant morphology, leaf microstructure and ultrastructure were observed, and the relative electrical conductivity (REL), malondialdehyde (MDA), soluble protein (SP), soluble sugar (SS), proline (Pro) content, net photosynthesis rate (Pn), intercellular carbon dioxide (Ci), and chlorophyll content were measured. The results showed that the most significant differences in plant morphology and survival rate between the strong cold\|tolerant line 2016TS(G)10 and the weak cold\|tolerant variety Tianyou 2288 were observed when exposed to -2℃. The physiological indicators of the two materials, including REL, MDA, SP, SS, Pro content, Pn, Ci, chlorophyll a, chlorophyll b content, and chlorophyll a/b, changed in a similar trend, but the SP and SS content of the strong cold\|tolerant line 2016TS(G)10 were 5.10% and 29.69% higher than those of in Tianyou 2288 (P≤0.01), respectively, and the REL and MDA content were 4.8% and 17.9% lower (P≤0.01). The chloroplast structure of 2016TS(G)10 remained intact, with a more accumulation of starch grains, while the chloroplasts of Tianyou 2288 were broken and degraded, with no presence of starch grains. After being recovered at room temperature for 48 hours, the 2016TS(G)10 exhibited a stronger recovery in plant morphology and Pn. Therefore, the cold tolerance of northern strong winter rapeseed should be evaluated under freezing stress ranging from 0℃ to -4℃. The stability and number of chloroplasts, especially the accumulation of starch granules, is an important factor affecting the cold resistance of winter rapeseed planted in northern China. |
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