| Genome\|wide identification of BnAR gene family and expression analysis under low temperature stress in Brassica napus L. |
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| DOI：10.7606/j.issn.1000-7601.2024.06.04 |
| Key Words: Brassica napus L. BnAR gene family bioinformatics expression pattern low temperature stress |
| Author Name | Affiliation | | GUO Zhiting | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | QIN Mengfan | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | LI Haodong | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | ZHAO Feixue | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | LI Xinru | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | DU Yuanyuan | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | BAO Shunjun | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | LI Hongyang | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | XU Jiali | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | HUANG Zhen | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| To understand the basic information, evolutionary relationship and the function of BnAR genes under cold stress in Brassica napus L., the BnAR gene family of B.napus were identified in this study, and the expression of family members under low temperature conditions were explored. The results showed that 14 BnAR genes were identified in B.napus. They were distributed on eight chromosomes and renamed as BnAR-1 to BnAR-14, dividing into ADC and DapDC subgroups. Promoter cis\|element analysis showed that the promoter region of BnAR genes contained various elements related to hormone and stress response. Intra\|group collinearity analysis of B.napus showed that 11 pairs of genes had collinearity relationship, and Ka/Ks analysis showed that the ratio of all homologous gene pairs were less than 0.5, indicating that the BnAR gene family was subjected to strict purification selection in the evolutionary process. Transcriptome data analysis indicated that all 14 BnAR genes responded to low temperature stress, among which four genes, BnAR-4, BnAR-5, BnAR-11 and BnAR-13 were strongly subjected to low temperature stress. The results of qRT-PCR were highly consistent with the transcriptome data, so these four genes could be used as candidate genes for subsequent functional verification. |
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