| Identification of the whole CNGC gene family in alfalfa and analysis of its regulatory patterns under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2025.01.06 |
| Key Words: tetraploid alfalfa CNGC gene family protein phosphorylation drought stress expression mode |
| Author Name | Affiliation | | QIU Yingde | College of Forestry, Gansu Agricultural University, Lanzhou,Gansu 730070, China | | LUO Yongzhong | College of Forestry, Gansu Agricultural University, Lanzhou,Gansu 730070, China | | QI Jianwei | College of Forestry, Gansu Agricultural University, Lanzhou,Gansu 730070, China | | ZHOU Xiaotong | College of Forestry, Gansu Agricultural University, Lanzhou,Gansu 730070, China | | MA Chao | College of Forestry, Gansu Agricultural University, Lanzhou,Gansu 730070, China | | YU Simin | College of Forestry, Gansu Agricultural University, Lanzhou,Gansu 730070, China |
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| Abstract: |
| Cyclic nucleotide\|gated channel proteins (CNGC) play an important role in Ca2+ signal transduction. Genome\|wide identification of CNGC gene family members in tetraploid alfalfa (Medicago sativa ‘Xinjiangdaye’) and analysis of their regulatory patterns under drought stress were carried out using bioinformatics and molecular biology research methods. The results revealed that the ‘Xinjiangdaye’ alfalfa genome contains 67 MsCNGC genes, unevenly distributed across 30 chromosomes. These genes were classified into five subfamilies, contain 20 Motifs, and exhibit various regulatory elements in their promoter regions that respond to environmental stress. A total of 28 MsCNGC genes were expressed in the leaves of ‘Xinjiangdaye’ alfalfa, and 26 of them were unevenly up\|regulated under drought stress, among which MsCNGC59 was the highest expressed and the most multiplicatively up\|regulated under drought stress. The proteins of MsCNGCs contained 29~120 phosphorylation sites, which could be phosphorylated by the LRR protein kinase, and the gene expression of MsLRRs and MsCNGCs showed a significant positive correlation. In addition, the expression levels of Ca2+ signal transduction\|related genes MsCaM, MsCML and MsCDPK were found to be significantly up\|regulated under drought stress. In summary, the response of tetraploid alfalfa MsCNGCs to drought stress included transcriptional regulation and protein phosphorylation regulation, which can improve the drought resistance of alfalfa under drought stress by participating in the Ca2+ signal transduction pathway. |
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