| Effects of drought stress on yield and expression of drought\|resistant genes in dryland winter wheat |
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| DOI：10.7606/j.issn.1000-7601.2025.01.07 |
| Key Words: dryland winter wheat early generations drought stress drought resistant genes variety screening |
| Author Name | Affiliation | | YANG Xiao | College of Agriculture and Biological Engineering, Longdong University, Qingyang, Gansu 745000, China
Collaborative Innovation Center for Longdong Dryland Crop Germplasm Improvement and Industrialization, Qingyang, Gansu 745000, China
Engineering Research Center for Germplasm Innovation and Application of Dryland Winter Wheat in Gansu Province, Qingyang, Gansu 745000, China | | CHEN Delai | College of Agriculture and Biological Engineering, Longdong University, Qingyang, Gansu 745000, China | | LIU Zicheng | College of Agriculture and Biological Engineering, Longdong University, Qingyang, Gansu 745000, China
Collaborative Innovation Center for Longdong Dryland Crop Germplasm Improvement and Industrialization, Qingyang, Gansu 745000, China
Engineering Research Center for Germplasm Innovation and Application of Dryland Winter Wheat in Gansu Province, Qingyang, Gansu 745000, China | | LI Jing | Dingxi Agricultural Technology Extension Center, Dingxi, Gansu 743000, China | | SHI Wanxi | College of Agriculture and Biological Engineering, Longdong University, Qingyang, Gansu 745000, China
Collaborative Innovation Center for Longdong Dryland Crop Germplasm Improvement and Industrialization, Qingyang, Gansu 745000, China
Engineering Research Center for Germplasm Innovation and Application of Dryland Winter Wheat in Gansu Province, Qingyang, Gansu 745000, China | | MENG Jianjun | College of Agriculture and Biological Engineering, Longdong University, Qingyang, Gansu 745000, China
Collaborative Innovation Center for Longdong Dryland Crop Germplasm Improvement and Industrialization, Qingyang, Gansu 745000, China
Engineering Research Center for Germplasm Innovation and Application of Dryland Winter Wheat in Gansu Province, Qingyang, Gansu 745000, China |
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| Abstract: |
| To investigate the relationship between the drought resistance of newly developed winter wheat strains in the drylands of eastern Gansu Province and their drought\|resistant functional genes, six new dryland winter wheat strains were selected as experimental materials. These were planted at the Xifeng Dry Farming Agricultural Experimental Station of Longdong University during the 2021-2022 and 2022-2023 growing seasons. Three water treatments were set up: rain\|fed without irrigation (CK), drought shelter to prevent rain (drought stress) and regulated irrigation. The yield was measured to evaluate their drought resistance, and the relative expression levels of drought\|related functional genes were analyzed. The results showed that the yield difference of 6 wheat strains showed inconsistent changes under water stress and 70% of the field capcity. The yield difference between two years of C (‘1576-2-0-2’) and D (‘15119-1-0-2’) was the smallest, and the differences of the other 4 strains were more than the average difference. In the rain-fed without irrigation experiment, the year 2022 was identified as a severe drought year. The yields of strains C and D ranked first and third among the six tested strains, and the drought resistance coefficients of 0.89 and 1.02, respectively. In 2023, a relatively wet year, the drought resistance coefficients for strains C and D were 0.77 and 1.12, indicating that these two strains were less sensitive to climate anomalies and had good drought resistance. The relative expression levels of six drought\|resistant functional genes were analyzed in the flag leaves of six wheat strains during the grain filling stage under drought stress using fluorescence quantitative PCR. The results showed the TaCRT-D gene had the highest expression level in these strains. The expression levels of Wdreb2, XTH-7A, WIip19, and TaCRT-D in strains C and D were higher than those in other strains. The field trial results align with the identification outcomes observed at the germination and seedling stages under drought stress simulated by a PEG-6000 hypertonic solution. They indicate that among the six lines, ‘1576-2-0-2’ and ‘15119-1-0-2’ exhibit strong drought resistance. Furthermore, these findings confirm that during drought\|resistant breeding of winter wheat, the expression of the drought\|resistance functional gene TaCRT-D in early flag leaves under drought stress during the grain\|filling stage can serve as an effective early\|generation selection index for drought resistance. |
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