| Isolation and identification of potato black rot Pectobacterium atrosepticum and indoor fungicide screening |
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| DOI：10.7606/j.issn.1000-7601.2025.01.25 |
| Key Words: potato black shank disease pathogen identification Pectobacterium atrosepticum bactericide |
| Author Name | Affiliation | | WANG Yue | Academy of Agriculture and Forestry Science of Qinghai University, Xining, Qinghai 810016, China
Scientific Observation and Experiment Station of Crop Pest in Xining in Ministry of Agriculture, Xining, Qinghai 810016, China
Key Laboratory of Agricultural Integrated Pest Management in Qinghai, Xining, Qinghai 810016, China | | MA Yongqiang | Academy of Agriculture and Forestry Science of Qinghai University, Xining, Qinghai 810016, China
Scientific Observation and Experiment Station of Crop Pest in Xining in Ministry of Agriculture, Xining, Qinghai 810016, China
Key Laboratory of Agricultural Integrated Pest Management in Qinghai, Xining, Qinghai 810016, China |
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| Abstract: |
| Using pathogen culture characteristics, pathogenicity verification, 16S rRNA gene sequence comparison, and specific primer amplification detection, suspected cases of potato black shank disease were isolated and identified from 16 towns in Haidong and Xining of Qinghai Province. The indoor virulence of seven fungicides against the pathogen causing potato black shank was evaluated using the growth rate method. From 68 suspected black shank samples, a total of 15 strains of Pectobacterium atrosepticum were successfully isolated and identified.The results of laboratory virulence test showed that the pathogenic bacteria had different inhibitory effects on 7 chemical agents. Among them, 0.3% tetramycin water, 46% copper hydroxide wettable powder, and 30% copper succinate wettable powder had the best antibacterial effect, with EC50 values of 1.355 μg·mL-1, 1.403 μg·mL-1 and 1.835 μg·mL-1, respectively. It was followed by 10% aqueous nanosilver, 3% mesorhizobium wettable powder and 40% copper quinoline suspension, with EC50 values of 106.429 μg·mL-1, 146.332 μg·mL-1 and 149.121 μg·mL-1, respectively. The sensitivity of pathogens to 10% difenoconazole aqueous dispersion granules was the worst. The EC50 value was 1 384.943 μg·mL-1. The results of this study provide scientific basis for rational selection of pesticides in production and field control of potato black shank disease. |
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