| Effects of ectomycorrhizal fungi on seedling growth and drought resistance of Taxus cuspidata |
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| DOI：10.7606/j.issn.1000-7601.2025.02.07 |
| Key Words: Taxus cuspidata ECMF draught stress seedling drought resistance |
| Author Name | Affiliation | | LI Qiuhong | School of Science, Eastern Liaoning University, Dandong, Liaoning 118003, China | | WANG Dandan | School of Science, Eastern Liaoning University, Dandong, Liaoning 118003, China | | ZHANG Yanwen | School of Science, Eastern Liaoning University, Dandong, Liaoning 118003, China |
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| Abstract: |
| To investigate the mechanism of ectomycorrhizal fungi (ECMF) in enhancing drought resistance of T. cuspidata seedlings, this study analyzed the effects of Cenococcum geophilum (Cr), Suillus granulatus (Se), and Suillus viscidus (Sb) on seedling infection rate, growth parameters, physiological indicators, and gene expression levels under normal watering (N), mild drought stress (L), moderate drought stress (M), and severe drought stress (S) conditions. Results showed that the control group (CK) had an infection rate of only 0.8%~1.2% with a decreasing trend as drought intensified, while Cr showed the highest infection rate (43.61%~60.56%), significantly higher than Se (41.09%~53.27%) and Sb (31.17%~34.85%). In terms of growth parameters, ECMF inoculation significantly improved seedling height, ground diameter, biomass, and root\|shoot ratio, with Cr and Se treatments increasing total biomass by 66.83% and 35.39% respectively under moderate drought stress. Regarding physiological indicators, ECMF inoculation significantly improved photosynthetic parameters and antioxidant capacity, with Cr treatment showing 40.04% and 126.42% increases in net photosynthetic rate and stomatal conductance respectively under moderate drought stress. For antioxidant enzyme activity, under mild, moderate, and severe stress, Cr treatment increased SOD activity by 66.85%, 92.33%, and 33.19%, while Se increased by 48.66%, 75.23%, and 31.45% respectively. Osmotic regulation substance content showed an initial increase followed by a decrease with increasing drought stress, with Cr, Se, and Sb treatments reaching 1.96, 1.83, and 1.52 times that of normal watering levels of proline content under moderate drought stress. At the gene expression level, the TcDREB gene in the Cr treatment group showed a 16.82% increase under moderate drought stress compared to the control group, however, there was no significant differences (P>0.05) between the Se and Sb treatment groups and the non\|inoculated groups. The research demonstrated that among the three ECMF species, the drought resistance effectiveness ranked as Cr>Se>Sb. Through various mechanisms, including enhanced photosynthetic capacity, a strengthened antioxidant system, and improved osmotic regulation, these fungi significantly increased the drought resistance of T. cuspidata seedlings and promoted their growth, thereby providing a valuable theoretical foundation for drought\|resistant seedling cultivation. |
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