| Overexpression of the cucumber CsAGO1c gene reduces drought tolerance in Arabidopsis thaliana |
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| DOI：10.7606/j.issn.1000-7601.2025.03.06 |
| Key Words: cucumber CsAGO1c gene overexpression Arabidopsis drought tolerance |
| Author Name | Affiliation | | GUAN Lili | College of Horticulture, Anhui Agricultural University, Hefei, Anhui 230036, China | | KUANG Yong | College of Horticulture, Anhui Agricultural University, Hefei, Anhui 230036, China | | XIAO Yi | College of Horticulture, Anhui Agricultural University, Hefei, Anhui 230036, China | | SUN Zhaolong | College of Horticulture, Anhui Agricultural University, Hefei, Anhui 230036, China | | GAN Defang | College of Horticulture, Anhui Agricultural University, Hefei, Anhui 230036, China |
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| Abstract: |
| CsAGO1c was cloned from the inbred line of cucumis variety ‘Xintaimici’, and its expression was analyzed under drought stress. An overexpression vector for CsAGO1c was constructed and used to transform Arabidopsis via Agrobacterium\|mediated transformation. The phenotypic and physiological changes of the CsAGO1c\|overexpressing plants were then studied under drought stress conditions. The results showed that the expression level of CsAGO1c in cucumber root first decreased and then increased. The expression level of CsAGO1c was down\|regulated by 57% at 6 h，down\|regulated by 64% at 12 h，and up\|regulated by 4.24 times at 24 h. The expression level of CsAGO1c in the leaves decreased first, then increased and then decreased, and the expression level of CsAGO1c increased 1.16 times at 18 h. Overexpressed Arabidopsis seeds germinated earlier than the wild type. The penetration test showed that 400 mmol·L-1 mannitol treatment significantly inhibited the growth of OE2 root length，which was 54% that of WT. The water loss rate of isolated leaves was 1.21, 1.27, and 1.25 times higher than that of the wild type (WT) at 3, 4, and 5 hours, respectively, showing a significantly greater loss compared to WT at all three time points. Additionally, under drought stress, the activities of superoxide dismutase and peroxidase in the overexpressing Arabidopsis lines were approximately 64% and 51% of those in WT, respectively, both significantly lower than WT levels. Meanwhile, the malondialdehyde content in OE2 was about three times higher than that of WT, indicating a marked increase. These findings suggest that heterologous expression of CsAGO1c reduces drought tolerance in Arabidopsis. This study provides valuable theoretical insight into the function of the CsAGO1c gene in cucumber and offers a foundation for breeding new drought\|resistant cucumber varieties. |
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