| Evaluation of cold resistance in one\|year\|old peach germplasm seedlings |
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| DOI：10.7606/j.issn.1000-7601.2025.04.03 |
| Key Words: peach germplasm resources early assessment of cold tolerance LT50 physiological index |
| Author Name | Affiliation | | LI Jie | Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China | | TIAN Qihang | Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China | | WANG Yu | Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China | | LIU Guojian | Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China | | WANG Zhaoyuan | Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China | | TIAN Zhendong | Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China | | CHANG Ruifeng | Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China | | LI Yonghong | Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China |
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| Abstract: |
| One\|year\|old seedlings of 21 peach germplasms, subjected to cold hardiness treatment at low temperatures, were used as experimental materials. To compare and analyze their cold tolerance, we calculated the lethal temperature (LT50) and recorded the field survival rates. Meanwhile, physiological indicators were measured, and the relationship between survival rate and cold resistance was analyzed. Then, we used LT50 and subordinate function, respectively, to clarify the cold tolerance classification of twenty\|one peach. Under cold stress, branches kept relatively high enzyme activity of phenylalanine ammonia\|lyase (PAL). Moreover, the high content of malondialdehyde (MDA), free proline (Pro) and soluble sugar (SS) was detected, and LT50 was relatively lower (range from -26.65℃ to -17.69℃). In the field experiment, the survival rate of the plants also decreased gradually with the decrease in temperature. When the temperature was below -20℃, varieties such as‘Zhonghuashoutao’declined particularly significantly, with the survival rate all below 50%. When the temperature dropped to -25℃, the survival rates of the varieties ‘Hangongzhu’, ‘Donghe 1’, and ‘Hunchun’ ranged between 80% and 90%, while those of the other varieties were all below 80%. When the temperature dropped to -30℃, the survival rate of all varieties fell to 0. By using the hierarchical cluster method, the cold resistance ability of 21 peach germplasm resources of young trees can be classified into five categories in sequence from extremely strong to weak: the first category was ‘Hangongzhu’, the categories of 2, 3, 4, and 5 respectively contain 3, 4, 9, and 4 varieties. Correlation analysis showed that the survival rate was significantly positively with comprehensive evaluation value (D), PAL activity, contents of Pro, and SS, and significantly negatively correlated with the LT50 and MDA content. Significant differences in cold resistance were observed among young peach trees. Following low\|temperature treatment, the PAL activity and the contents of MDA, Pro, and SS showed strong correlations with cold resistance, indicating they can serve as key indicators for assessing cold tolerance in young peach germplasm. |
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