Effects of exogenous organic materials on soil aggregates, enzyme activities, and microbial communities in newly built agricultural fields
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DOI:10.7606/j.issn.1000-7601.2025.04.11
Key Words: newly built high\|standard farmland  natural humus material  soil quality  soil aggregates  soil enzyme activity  microbial communities
Author NameAffiliation
DING Zhaoyun Gansu Academy of Agri\|Engineering Technology, Lanzhou, Gansu 730000, China 
SU Cuicui Gansu Academy of Agri\|Engineering Technology, Lanzhou, Gansu 730000, China 
ZHANG Jing Gansu Academy of Agri\|Engineering Technology, Lanzhou, Gansu 730000, China 
ZHOU Yanfang Gansu Academy of Agri\|Engineering Technology, Lanzhou, Gansu 730000, China 
LIU Qiang Gansu Academy of Agri\|Engineering Technology, Lanzhou, Gansu 730000, China 
DENG Chaochao Gansu Academy of Agri\|Engineering Technology, Lanzhou, Gansu 730000, China 
WANG Zhenlong Gansu Academy of Agri\|Engineering Technology, Lanzhou, Gansu 730000, China 
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Abstract:
      In order to explore new ways to rapidly enrich the soil of newly built high\|standard farmland, we investigated the effects of different amounts of organic materials on the stability of aggregates, enzyme activities, and microbial communities in newly constructed farmland with loess soil through a two\|year localization experiment. The experimental design involved four distinct treatments: an adjacent high\|yield field without organic materials, conventional fertilization as a control (CK), and three treatments that incorporated natural humus material (4 500 kg·hm-2) + bio-organic fertilizer (1 500 kg·hm-2) + bio-stimulant + conventional fertilization (T1), natural humus material (9 000 kg·hm-2) + bio-organic fertilizer (3 000 kg·hm-2) + bio-stimulant + conventional fertilization (T2), and natural humus material (13 500 kg·hm-2) + bio\|organic fertilizer (4 500 kg·hm-2) + bio-stimulant + conventional fertilization (T3). The results showed that moderate exogenous organic materials contributed to the formation of large aggregates and the increment of aggregate stability in new agricultural soils. Compared with CK, T2 and T3 treatments significantly increased the average weight diameter of agglomerates, geometric mean diameter, content of >0.25 mm agglomerates R0.25, and the proportion of >2 mm agglomerates increased by 2.26% to 34.64%, and the activities of soil cellulase, sucrase, and peroxidase were elevated by 17.39% to 18.44%, 19.42% to 86.97%, and 75.37% to 81.02%, respectively. Meanwhile, T3 significantly decreased fractal dimension and polyphenol oxidase activity. The high\|throughput sequencing analysis showed that the Ace index of fungal community was significantly increased in T2, and the relative abundance of Ascomycota increased to 69.52% compared with CK. Correlation analysis revealed that fungal community structure and diversity were mainly influenced by soil sucrase and polyphenol oxidase activities, bacterial community structure and diversity were mainly influenced by >2 mm soil aggregates and peroxidase activity, and the number of microbial markers for both fungi and bacteria was greatest in T2. Therefore, from the perspectives of soil quality improvement and cost savings, natural humus material (9 000 kg·hm-2) + bio\|organic fertilizer (3 000 kg·hm-2) + bio\|stimulant + conventional fertilizer application can significantly improve the soil aggregate structure and soil enzymes activity of newly\|built farmland on loess soils and positively contribute to the enhancement of microbial diversity and community clustering in the short term.