| Effects of exogenous 6-BA on physiological characteristics and related gene expression of soybean seeds and seedlings under saline\|alkali stress |
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| DOI：10.7606/j.issn.1000-7601.2025.05.05 |
| Key Words: soybean 6-BA saline\|alkali stress seed germination seedling anti\|stress physiology |
| Author Name | Affiliation | | LI Weijia | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
National Coarse Cereals Engineering Research Center Crop Germplasm Resource Innovation Laboratory, Daqing, Heilongjiang 163319, China | | YU Miao | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | WEI Jiayi | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | MA Shile | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | ZHOU Yang | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | WU Xu | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | GUAN Weiming | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | DU Jidao | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
Heilongjiang Saline\|alkali Land Lmprovement Engineering Technology Research Center, Daqing, Heilongjiang 163319, China
National Coarse Cereals Engineering Research Center Crop Germplasm Resource Innovation Laboratory, Daqing, Heilongjiang 163319, China | | DU Yanli | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
Heilongjiang Saline\|alkali Land Lmprovement Engineering Technology Research Center, Daqing, Heilongjiang 163319, China
National Coarse Cereals Engineering Research Center Crop Germplasm Resource Innovation Laboratory, Daqing, Heilongjiang 163319, China |
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| Abstract: |
| This study investigated the mechanism of exogenous 6-benzyladenine (6-BA) in alleviating salt\|alkali stress on soybean seed germination and seedling growth using two soybean cultivars, ‘Henong 95’ and ‘Niuyanjing’. Through seed germination and seedling growth experiments, the effects of salt\|alkali stress and 6-BA treatment on germination parameters, growth indicators, antioxidant systems, endogenous hormone regulation, and related gene expression were compared. The results showed that salt\|alkali stress induced ROS accumulation in soybean seeds and seedlings, causing oxidative damage and significant reductions in radicle length (78.34%~81.00%), dry mass (52.31%~63.40%), radicle volume (67.37%~80.62%), radicle surface area (85.15%~85.20%), and root diameter (15.49%~45.52%) compared to controls. Application of 60 μmol·L-1 6-BA enhanced salt\|stress tolerance by regulating the antioxidant defense system and ROS accumulation, and improved germination rate (33.33%), germination potential (7.32%~51.85%), germination index (20.76%~32.27%), and vigor index (2.80%~49.09%) in both cultivars. Exogenous 6-BA modified endogenous hormone levels (tZ, GA3, IAA, and ABA), increasing their contents by 7.85%~16.37% in ‘Henong 95’ while decreasing them by 5.56%~12.77% in ‘Niuyanjing’. This treatment alleviated radicle growth inhibition by regulating cytokinin metabolism and signaling\|related genes, significantly improving radicle dry matter mass (88.71%~145.55%), root volume (117.90%~465.00%), root surface area (78.64%~98.87%), and root diameter (124.82%~235.48%). Compared to salt\|alkali treatment alone, 6-BA application increased leaf color value (77.90%~89.62%) and stomatal conductance (118.85%~125.67%), effectively enhancing photosynthetic capacity and antioxidant activity in seedlings. These improvements promoted underground nodule formation and root growth, resulting in increased root length (1.55%~17.00%) and dry matter mass (70.43%~75.36%). |
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