Effects of ridge and furrow mulching and phosphorus application on the ecological stoichiometry of soil\|plant\|microorganism system in alfalfa field
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DOI:10.7606/j.issn.1000-7601.2025.05.15
Key Words: ridge and furrow mulching  phosphorus application  Medicago sativa L.  soil\|plant\|microorganism system  ecological stoichiometry
Author NameAffiliation
XU Qiuyun College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai 810016, China 
ZHOU Weidi College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai 810016, China
Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai 810016, China 
HAN Chenglong State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai 810016, China 
GU Yanjie College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai 810016, China 
LI Fengmin College of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 
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Abstract:
      From 2011 to 2016, the alfalfa artificial grassland was used as the research object in Zhonglianchuan village, Yuzhong County, Gansu Province. The randomized block of split\|plot design was used. Two mulching methods (no mulching with flat planting and mulching with ridge and furrow) and four phosphorus application gradients (P0: no P applied, P1: apply 9.73 kg·hm-2 phosphorus, P2: apply 19.3 kg·hm-2 phosphorus, and P3: apply 28.9 kg·hm-2 phosphorus) were set up. During the period, soil and aboveground plant samples were collected every year to analyze the C, N, and P content of the soil\|plant\|microorganism system and their ecological stoichiometric characteristics. The results showed that, (1) In addition to high P treatment (P3), plastic film mulch with ridge and furrow averagely increased soil C, N, C∶N, C∶P, N∶P, soil microbial biomass C contents, C∶N, and C∶P by 39.3%, 27.1%, 13.5%, 55.3%, 49.8%, 57.6%, 39.6%, and 43.3%, respectively, compared to no mulching with flat planting. Soil available P under mulching with ridge and furrow averagely decreased by 13.9%, compared to no mulching with flat planting. (2) Soil C, C∶N, plant N, soil microbial biomass C, C∶N, and C∶P increased with increasing P levels in no mulching with flat planting but increased initially and then decreased with the increasing P levels under mulching with ridge and furrow. (3) Plant C∶N and soil microbial biomass nitrogen (MBN) in P1, P2 and P3 treatments decreased by 2.94%, 7.02%, 11.5% and 8.62%, 11.5%, 19.1%, respectively, compared to P0 in no plastic film mulch with flat planting. Plant C∶N and MBN in P1 treatment decreased by 10.0% and 24.3% respectively compared to P0, while in P3 treatment increased by 8.20%, 5.93% and 27.4%, 9.92%, respectively, compared to P1 and P2 under mulching with ridge and furrow. (4) P fertilization respectively increased soil, plant, and soil microbial biomass P by 67.2% to 164.0%, 14.0% to 34.3%, and 19.2% to 87.0%, and respectively decreased soil C∶P and N∶P, plant C∶P and N∶P, and soil microbial biomass N∶P by 29.5% to 51.5%, 30.1% to 54.3%, 13.2% to 28.3%, 6.71% to 19.8%, and 29.1% to 52.7%. (5) With the extension of planting years, by 2016, soil C, N, C∶N, plant N : P, and soil microbial biomass C∶P had cumulatively increased by 49.3%、33.9%、11.3%、77.6% , and 126.0%, respectively compared to 2011, while plant phosphorus content had cumulatively decreased by 51.3%. ]In summary, the growth of alfalfa and soil microorganisms was limited by N deficiency under mulching with ridge and furrow and a high amount of P fertilizer. This management practice accelerated the decomposition of soil organic matter, which was not conducive to maintaining the growth of alfalfa and soil fertility in the semi\|arid region of the Loess Plateau. Therefore, when the amount of P applied under mulching with ridge and furrow is higher than 22.7 kg·hm-2, appropriate nitrogen fertilizer should be supplied.