Isolation and identification of endophytic salt\|alkali tolerant growth\|promoting bacteria from soybean roots and their growth\|promoting functions
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DOI:10.7606/j.issn.1000-7601.2025.05.24
Key Words: soybean  root endophytic bacteria  salt\|alkai tolerance  isolation and identification  growth\|promoting function
Author NameAffiliation
XIA Yuqiao Heilongjiang Bayi Agricultural University, Key Laboratory of Low\|carbon Green Agriculture in Northeastern Plain, Ministry of Agriculture and Rural Affairs P.R. China, Daqing, Heilongjiang 163319, China 
JING Ruiyong Heilongjiang Bayi Agricultural University, Key Laboratory of Low\|carbon Green Agriculture in Northeastern Plain, Ministry of Agriculture and Rural Affairs P.R. China, Daqing, Heilongjiang 163319, China 
JIANG Xue Heilongjiang Bayi Agricultural University, Key Laboratory of Low\|carbon Green Agriculture in Northeastern Plain, Ministry of Agriculture and Rural Affairs P.R. China, Daqing, Heilongjiang 163319, China 
WANG Liyan Heilongjiang Bayi Agricultural University, Key Laboratory of Low\|carbon Green Agriculture in Northeastern Plain, Ministry of Agriculture and Rural Affairs P.R. China, Daqing, Heilongjiang 163319, China 
GUO Yongxia Heilongjiang Bayi Agricultural University, Key Laboratory of Low\|carbon Green Agriculture in Northeastern Plain, Ministry of Agriculture and Rural Affairs P.R. China, Daqing, Heilongjiang 163319, China 
YU Zhenhua Institute of Northeast Geography and Agricultural Ecology, Chinese Academy of Sciences, Key Laboratory of Agricultural Ecology in the Black Soil Region, Harbin, Heilongjiang 150081, China 
LIU Junjie Institute of Northeast Geography and Agricultural Ecology, Chinese Academy of Sciences, Key Laboratory of Agricultural Ecology in the Black Soil Region, Harbin, Heilongjiang 150081, China 
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Abstract:
      In order to explore the endophytic bacteria resources of soybean roots in soda saline\|alkali land, the soybean roots in Daqing soda saline\|alkali land were used as materials. The endophytic bacteria in soybean roots were isolated, purified, and identified by tissue grinding, plate streaking, and PCR sequencing, and their phosphate solubilization, nitrogen fixation, siderophore production, ACC deaminase, IAA ability, and soda saline\|alkali tolerance were determined. The soybean germination test was carried out under soaking conditions. The results showed that the three screened endophytic bacteria that had the functions of phosphate solubilization, nitrogen fixation, siderophore production, plant hormone IAA production, ACC deaminase production, and tolerance to soda saline\|alkali (100~150 mmoL·L-1 NaHCO3 and Na2CO3, molar ratio of 9∶1; pH 8.8) were identified as Bacillus siamensis DC.24, Bacillus wiedmannii DM57, and Pseudomonas hibiscicola DC-2. In the germination test, the number of root tips, root length, root surface area, and root volume of soybean were significantly increased by soaking seeds with three strains of endophytic bacteria. Inoculation of the three endophytic bacteria into the ‘Hefeng 50’ soybean variety resulted in an increase of 169.7%, 221.2%, and 172.7% in root tip number, 52.8%, 63.0%, and 29.8% in root length, 139.9%, 125.6%, and 96.9% in root volume, and 89.2%, 83.6%, and 58.7% in root surface area, respectively. In the ‘Longken 310’ variety, the number of root tips increased by 758.8%, 329.4%, and 341.1%, root length increased by 101.1%, 52.2%, and 50.0%, root volume increased by 129.4%, 82.3%, and 70.5%, and root surface area increased by 92.6%, 51.2%, and 41.4%, respectively. This indicated that the three root endophytic bacteria have growth\|promoting functions and need to be developed for soda saline\|alkali soil microbial fertilizer.