| Screening and identification of upland cotton germplasm with tolerance to compound salt stress at germination and three\|leaf stages |
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| DOI：10.7606/j.issn.1000-7601.2025.06.02 |
| Key Words: upland cotton (Gossypium hirsutum L.) germplasm compound salt stress germination stage three\|leaf stage |
| Author Name | Affiliation | | LUO Xiheng | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | ZHANG Tong | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | WU Yanqing | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | WANG Peiyan | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | TIAN Ziyi | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | TANG Qing | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | LIU Jiao | Key Laboratory of Saline\|Alkali Soil Improvement and Utilization (Saline\|Alkali Land in Arid and Semi\|Arid Regions) of Ministry of Agriculture and Rural Affairs, Institute of Soil Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumuqi, Xinjiang 830091, China | | YANG Yanlong | Institute of Cash Crop Research, Xinjiang Academy of Agricultural Sciences, Urumuqi, Xinjiang 830091, China | | HAN Huanyong | Xinjiang Academy of Agricultural and Reclamation Science Cotton Research Institute， Shihezi, Xinjiang 832099, China | | LEI Zhangying | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | HE Daohua | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| To screen for cotton genotypes with tolerance to composite salt stress, 166 cotton genotypes were used as experimental materials and subjected to compound salt stress at concentrations of 0.15 mol·L-1 and 0.30 mol·L-1. During the germination stage, genotypes were evaluated using comprehensive ranking and fuzzy membership function methods to identify those with greater tolerance. The selected genotypes, along with the control varieties ‘Zhong 9807’ (salt\|tolerant) and ‘Xinong 606’ (salt\|sensitive), were grown to the three\|leaf stage and subjected to compound salt stress. The measurements of leaf morphology, relative chlorophyll content, gas exchange parameters, and chlorophyll fluorescence were conducted to further assess their tolerance. The results showed that seed germination and embryo root growth were significantly inhibited under 0.15 mol·L-1 compound salt\|alkali stress in most genotypes. Specifically, the germination vigor, germination rate, germination index, fresh weight, and embryo root length decreased by 72.558%, 63.542%, 74.737%, 52.790%, and 17.080%, respectively, compared with the control group treated with distilled water. However, 32 genotypes screened using germination traits showed relatively mild inhibition. At 0.30 mol·L-1 concentration, the inhibitory effects were significantly greater than those observed at 0.15 mol·L-1, with five measured traits showing reductions of 96.247%, 90.909%, 95.389%, 98.097%, and 97.602%, respectively. Through comprehensive evaluation, two compound salt\|tolerant genotypes, ‘De 93-04’ (ID: X170743) and ‘4SA-805’ (ID: X170523), were ultimately identified. Three\|leaf stage evaluations confirmed that these two genotypes maintained larger leaf areas and higher photosynthetic activity than the controls under high\|concentration composite salt stress, indicating their strong tolerance at the seedling stage. |
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