| Bioinformatics analysis of VrWRKY50 gene and expression analysis of prohexadione\|calcium alleviating saline\|alkali stress in mung bean |
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| DOI：10.7606/j.issn.1000-7601.2025.06.06 |
| Key Words: mung bean VrWRKY50 gene phylogeny saline\|alkali stress prohexadione\|calcium gene expression |
| Author Name | Affiliation | | PANG Yuanyuan | College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163000, China | | LI Chen | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163000, China | | JIANG Haipeng | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163000, China
Heilongjiang Province Plant Growth Regulator Engineering Technology Research Center, Daqing, Heilongjiang 163000, China | | WANG Qingyan | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163000, China
Heilongjiang Province Plant Growth Regulator Engineering Technology Research Center, Daqing, Heilongjiang 163000, China | | LIANG Xilong | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163000, China
Heilongjiang Province Plant Growth Regulator Engineering Technology Research Center, Daqing, Heilongjiang 163000, China | | FANG Shumei | College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163000, China
Heilongjiang Province Plant Growth Regulator Engineering Technology Research Center, Daqing, Heilongjiang 163000, China |
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| Abstract: |
| To explore the role of VrWRKY50 gene in mung bean response to saline\|alkali stress, analyze its protein structure and expression pattern under salt\|alkali stress and prohexadione\|calcium spray, in this study, 17 WRKY50 homologous proteins were analyzed by bioinformatics. qRT-PCR was used to determine the expression pattern of the VrWRKY50 gene in mung bean under saline\|alkali stress and prohexadione\|calcium spray. The results showed that all 17 WRKY50 proteins were hydrophilic proteins, and Motif 1 was the main conserved motif of WRKY50. The WRKY50 gene contained 3~4 exons, and the promoter region contained 9 cis\|acting elements related to growth and development, stress response, hormone response, etc. The protein homology of mung bean VrWRKY50 and adzuki bean VaWRKY50 was the highest. Salt and alkali stress significantly up\|regulated VrWRKY50 gene expression in mung bean, increasing 13.91-fold (6th day) and 7.15-fold (8th day) in leaves and roots, respectively. The peak time of VrWRKY50 gene expression was advanced to 1 day by spraying prohexadione\|calcium. The expression levels in leaves and roots were 47.70 times and 3.52 times those in the saline\|alkali control, respectively. |
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