| Identification of HD-Zip gene family in Prunusavium L. and expression analysis under stress conditions |
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| DOI：10.7606/j.issn.1000-7601.2025.06.08 |
| Key Words: Prunusavium L. HD-Zip transcription factor gene expression abiotic stress |
| Author Name | Affiliation | | BA Guang | College of Bioengineering and Technology, Tianshui Normal University, Tianshui, Gansu 741000, China | | GONG Yanjing | College of Bioengineering and Technology, Tianshui Normal University, Tianshui, Gansu 741000, China | | LIU Haiyan | College of Bioengineering and Technology, Tianshui Normal University, Tianshui, Gansu 741000, China | | DENG Fei | College of Bioengineering and Technology, Tianshui Normal University, Tianshui, Gansu 741000, China | | WANG Hongming | College of Bioengineering and Technology, Tianshui Normal University, Tianshui, Gansu 741000, China |
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| Abstract: |
| Homodomain\|leucine zipper (HD-Zip) proteins are essential for regulating plant growth and development and responding to adversity stress. Bioinformatics methods were used to identify the sweet cherry (Prunusavium L.) HD-Zip gene family members and analyze their physicochemical properties, secondary structure, conserved motifs, phylogeny, promoter elements, and gene structure. Based on the transcriptome data, the HD-Zip family members were mined for tissue specificity and expression patterns under stress conditions; meanwhile, the expression of HD-Zip genes in different tissues of sweet cherry was detected by qRT-PCR. The results showed that there are 15 members of the HD-Zip family, coding for amino acids ranging from 127 aa to 837 aa, and most of the members are predicted to be localized in the nucleus. The HD-Zip family proteins were hydrophilic and unstable proteins, the structure of the HD-Zip gene was relatively complex, the number of exons ranged from 1 to 18, and different conserved motifs were distributed in different family members. The phylogenetic tree showed that the HD-Zip family members were divided into four subfamilies, and the members of the same subfamily had similar structural composition and conserved motifs. There were cis\|acting elements in the promoter region of HD-Zip genes for phytohormones such as growth hormone, gibberellin, and jasmonic acid, as well as for a variety of adversity stresses. The transcriptome results showed that PavHB7A was specifically expressed in fruits and pedicels, and the expression level of PavHB7B in flowers was significantly higher than that in other tissues, up to 200 times. The expression level of PavHB15 in young shoots was 27 times higher than that in mature leaves. qRT-PCR results showed that HD-Zip genes were differentially expressed in buds, roots, stems, leaves, and flowers. After drought treatment, the expression of PavHB7A was up\|regulated in the roots and leaves of ‘CDR-1’, which increased by 665.6% and 365.9%, respectively, indicating that the PavHB7A gene was involved in the response process of drought stress. |
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