| Identification of the DOF gene family in quinoa (Chenopodium quinoa) and analysis of expression patterns regulated by nitric oxide (NO) under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2026.01.04 |
| Key Words: quinoa DOF gene family NO (nitric oxide) drought stress bioinformatics expression pattern |
| Author Name | Affiliation | | WANG Xuewei | College of Life Science and Technology, Gansu Agriculture University, Lanzhou, Gansu 730070, China | | WANG Baoqiang | College of Life Science and Technology, Gansu Agriculture University, Lanzhou, Gansu 730070, China | | ZHU Xiaolin | College of Life Science and Technology, Gansu Agriculture University, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agriculture University, Lanzhou, Gansu 730070, China | | GUI Jinfeng | College of Life Science and Technology, Gansu Agriculture University, Lanzhou, Gansu 730070, China | | CHEN Lifei | College of Life Science and Technology, Gansu Agriculture University, Lanzhou, Gansu 730070, China | | WEI Xiaohong | College of Life Science and Technology, Gansu Agriculture University, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agriculture University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| DOF (DNA binding with one zinc finger) is a plant\|specific transcription factor that plays a crucial role in plants’ response to abiotic stress. Through bioinformatics methods, the DOF gene family members in quinoa were identified genome\|wide, and the expression patterns of this gene family under drought stress regulated by exogenous NO were analyzed using qRT-PCR technology. The results showed that a total of 25 family members of the quinoa DOF gene family were identified, with amino acid lengths ranging from 143 to 558 aa, molecular weights ranging from 16 079.9 to 59 102.2 Da, isoelectric points ranging from 4.52 to 9.60, hydrophobic indices less than 2, and all predicted to be located in the nucleus. Phylogenetic analysis divided the DOF gene family into five subfamilies. The 25 DOF genes were evenly distributed on 13 chromosomes, with 6 pairs of tandem repeat genes. There were 31 cis\|acting elements related to light response, tissue specificity, stress response, and plant hormones in the promoter region. DOF5.3 was the core node of the protein interaction network. Expression analysis in different tissues and organs indicated that CqDOFs were expressed in all quinoa tissues, with stronger expression in flowers and leaves. Under different abiotic stress conditions, most CqDOFs genes responded strongly in roots. qRT-PCR analysis showed that under drought stress regulated by exogenous NO, the relative expression levels of 16 CqDOFs genes significantly increased. Among them, the expression level of CqDOF10 under drought stress regulated by NO was 215.3 times that under drought stress alone, indicating that CqDOFs genes play an important role in the regulatory mechanism of quinoa’s response to drought stress, and exogenous NO can effectively increase the expression of genes under drought stress. |
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