| Identification and analysis of the YTH gene family and its response to drought stress in potato |
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| DOI：10.7606/j.issn.1000-7601.2026.01.05 |
| Key Words: potato drought stress YTH gene family genome\|wide analysis expression pattern m6A methylation |
| Author Name | Affiliation | | XIAO Junfei | Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730000, China | | QIAO Yan | Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730000, China； School of Agriculture and Bioengineering, Longdong University, Qingyang, Gansu 745000, China； Collaborative Innovation Center for Longdong Dryland Crop Germplasm Improvement & Industrialization, Qingyang, Gansu 745000, China | | REN Panrong | Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730000, China； School of Agriculture and Bioengineering, Longdong University, Qingyang, Gansu 745000, China | | QIN Xuexue | School of Agriculture and Bioengineering, Longdong University, Qingyang, Gansu 745000, China；Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration, Qingyang, Gansu 745000, China | | YANG Fang | School of Agriculture and Bioengineering, Longdong University, Qingyang, Gansu 745000, China； Collaborative Innovation Center for Longdong Dryland Crop Germplasm Improvement & Industrialization, Qingyang, Gansu 745000, China | | QI Weiliang | School of Agriculture and Bioengineering, Longdong University, Qingyang, Gansu 745000, China； Collaborative Innovation Center for Longdong Dryland Crop Germplasm Improvement & Industrialization, Qingyang, Gansu 745000, China；Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration, Qingyang, Gansu 745000, China | | WANG Fang | Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730000, China |
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| Abstract: |
| YTH proteins are a class of proteins containing the YTH domain, which can specifically bind RNA, participate in N6-methyladenosine (m6A) modification, and play a key role in biotic and abiotic stress responses. Preliminary work in this study identified 5 drought\|responsive YTH genes via transcriptome sequencing. We identified 21 YTH domain\|containing genes based on the HMMER model using the potato variety of ‘Favorita’ as the experimental material to elucidate their drought response mechanisms. By integrating bioinformatics analyses (chromosomal localization, gene structure, phylogenetic relationships, and cis\|element prediction) with expression validation under drought stress, their features and expression patterns were characterized. Additionally, protein\|protein interaction (PPI) networks were constructed using the STRING database to reveal functional associations. The results showed that these YTH genes were distributed across 5 chromosomes. Among them, the 5 drought\|responsive genes were identified as StYTH1.1, StYTH1.3, StYTH1.4, StYTH1.5, and StYTH2.1. Furthermore, structural and conserved motif losses were observed in StYTH3.1 and StYTH4.1. Phylogenetic tree and gene structure analyses revealed that the potato YTH family could be classified into 3 subfamilies, and collinearity analysis indicated that gene duplication events drove the evolution of the potato YTH family. The phylogenetic tree showed that potato YTH genes were more closely related to those of tomato. Additionally, drought\|inducible MYB binding sites and multiple plant hormone\|related functional elements were detected in StYTH 1.1/1.2/1.3/1.4/1.5/3.1/6.1/6.2/8.1. The STRING database predicted 20 partner proteins for StYTH proteins, which, together with the 5 drought\|responsive StYTH proteins, formed a complex protein interaction network primarily involved in regulating mRNA splicing modification, biotic and abiotic stress responses, and signaling pathways mediated by abscisic acid (ABA), salicylic acid (SA), and other plant hormones. |
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