| Growth and physiological effects of foliar application of VDAL on alleviating cinnamic acid stress in fava bean seedlings |
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| DOI：10.7606/j.issn.1000-7601.2026.01.07 |
| Key Words: fava bean VDAL (Verticillium dahliae Asp-f2 like protein） cinnamic acid (CA) autotoxicity continuous cropping obstacle |
| Author Name | Affiliation | | LIU Xiujuan | College of Horticulture, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | YANG Wenhuan | College of Horticulture, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | CAO Jinping | College of Horticulture, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Yuping | College of Horticulture, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| To explore the alleviation effects and appropriate application concentration of foliar application of Verticillium dahliae Asp-f2 like protein (VDAL) on the damage to fava bean seedlings under autotoxicity stress induced by cinnamic acid (CA), the potted seedlings of ‘Qingcan 13’ were used as the experimental material, and different concentrations of VDAL (1.0, 1.5, 2.0, 2.5 mg·L-1, and 3.0 mg·L-1) were set for foliar application by using 0.9 mmol·L-1 CA to simulate the autotoxicity in continuous cropping obstacles. The growth and physiological responses of the fava bean seedlings were investigated. The results indicated that CA stress significantly inhibited the growth of faba bean seedlings, and foliar spraying of 2.5 mg·L-1 DAL could effectively alleviate its toxic effects. Compared with the CA treatment, 2.5 mg·L-1 VDAL significantly increased the plant height, leaf area, shoot dry biaomass and root dry biaomass of faba bean seedlings by 104.88%, 58.39%, 115.71% and 103.85%, respectively. In terms of photosynthetic characteristics, the contents of leaf total chlorophyll and carotenoids, as well as net photosynthetic rate, transpiration rate and stomatal conductance under 2.5 mg·L-1 VDAL treatment were increased by 73.88%, 136.82%, 59.03%, 596.25% and 132.68%, respectively. At the physiological index level, 2.5 mg·L-1 VDAL significantly decreased the intercellular CO2 concentration, malondialdehyde content, and hydrogen peroxide content by 31.04%, 40.68% and 35.43%, respectively, while it increased the contents of proline, soluble sugar and soluble protein, as well as the activities of superoxide dismutase, peroxidase, catalase and ascorbate peroxidase by 73.18%, 27.08%, 33.54%, 16.99%, 145.58%, 102.82% and 96.20%, respectively. In conclusion, foliar spraying with appropriate concentration of VDAL could improve the photosynthetic rate, osmotic regulation ability and antioxidant capacity of faba bean seedlings under cinnamic acid stress, effectively alleviate the continuous cropping obstacle, with the optimal effect observed at a concentration of 2.5 mg·L-1. |
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