Effects of nitrogen fertilizer reduction and biochar application on photosynthetic characteristic and yield of intercropped maize
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DOI:10.7606/j.issn.1000-7601.2026.01.11
Key Words: maize intercropping fresh peas  nitrogen fertilizer reduction  biochar  photosynthetic characteristics  yield  soil nitrogen
Author NameAffiliation
WANG Xiaoli State Key Laboratory of Arid Land Crop Science, Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
NAN Yunyou State Key Laboratory of Arid Land Crop Science, Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
LI Yue State Key Laboratory of Arid Land Crop Science, Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
SHAO Juan State Key Laboratory of Arid Land Crop Science, Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
CHEN Han State Key Laboratory of Arid Land Crop Science, Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
ZHANG Huanhuan State Key Laboratory of Arid Land Crop Science, Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
CHAI Qiang State Key Laboratory of Arid Land Crop Science, Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
YIN Wen State Key Laboratory of Arid Land Crop Science, Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
HU Falong State Key Laboratory of Arid Land Crop Science, Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
FAN Zhilong State Key Laboratory of Arid Land Crop Science, Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
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Abstract:
      An experiment was conducted in the Hexi Oasis Irrigation Region from 2022 to 2023. The main plots consisted of maize intercropped with fresh peas (IM) and monoculture maize (SM), while the subplots included the local conventional nitrogen application rate (N1, 360 kg·hm-2) and reduction(30%)in the application rate (N2, 250 kg·hm-2). The sub\|subplots involved biochar application (C) and no biochar application. The study examined variations in net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), canopy coverage (CC), leaf stay\|greenness (SG), and dry matter accumulation (DMA) of maize with different treatment. These parameters were analyzed in conjunction with soil organic carbon (SOC), nitrate nitrogen (NO-3-N), and ammonium nitrogen (NH+4-N) content to explore the relationships between photosynthetic characteristic and grain yield (GY) of intercropped maize. The results showed that the Pn, Tr, Gs, and CC of intercropping maize were significantly increased by 2.6%-4.1%, 52.1%-55.1%, 8.6%-10.5% and 2.1%-2.3%, respectively, compared with monocropping maize (P<0.05). Nitrogen reduction by 30% reduced the Pn, Tr, Gs, and CC of maize, decreasing by 4.3%-6.5%, 21.5%-28.3%, 12.9%-15.6%, and 22.2%-24.3%, respectively, while the application of biochar made the photosynthetic indexes of intercropping maize not significantly different between N2 and N1. The DMA and GY of intercropping maize were significantly increased by 6.9%-7.9% and 18.6%-20.8% (P<0.05), respectively, compared with monoculture maize. Nitrogen reduction of 30% significantly reduced DMA and GY of maize, but the application of biochar significantly increased DMA and GY of maize by 11.3%-19.7% and 8.7%-17.5%, respectively, and there was no significant difference in DMA and GY between N2 and N1. The contents of SOC, NO-3-N and NH+4-N in intercropping maize belt were significantly higher than those in monoculture maize belt (P<0.05), increasing by 5.9%-7.9%,3.1%-7.2%, and 7.1%-10.8%, respectively, while the application of biochar further increased the contents of SOC, NO3-N, and NH+4-N in the intercropping maize belt under N2, so that there was no significant difference between N2 and N1, with increases of 6.4%-7.3%, 25.5%-26.8%, and 27.4%-27.9%, respectively. The soil NH+4-N content in the intercropped maize strip with reduced 30% nitrogen application was enhanced by biochar application, which subsequently improved canopy coverage and the stay\|green characteristic of intercropped maize. That improvement led to enhanced leaf photosynthetic parameters and increased dry matter accumulation, ultimately resulting in greater grain yield. Therefore, the integrated practice of biochar application in maize intercropped with fresh peas with reduced 30% nitrogen application rate can be recommended as a sustainable production pattern for nitrogen\|efficient maize cultivation in arid oasis irrigation regions.