| Adaptation analysis of Arabidopsis thaliana overexpressing Haloxylon ammodendron HaFT-1 gene under heat and drought cross\|stress |
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| DOI：10.7606/j.issn.1000-7601.2026.02.16 |
| Key Words: Haloxylon ammodendron HaFT-1 gene Arabidopsis thaliana overexpression heat and drought cross\|stress |
| Author Name | Affiliation | | WANG Xu | College of Life Sciences, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China | | LIU Yuanyuan | College of Life Sciences, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China | | GU Jun | College of Life Sciences, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China | | WANG Yameng | College of Life Sciences, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China | | CHEN Jianru | College of Life Sciences, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China | | ZHANG Hua | College of Life Sciences, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China | | WANG Bo | College of Life Sciences, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China |
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| Abstract: |
| To explore the function of the HaFT-1 protein in the cross\|stress tolerance of heat and drought stress, Haloxylon ammodendron seedlings and HaFT-1 overexpressing Arabidopsis thaliana were used as materials. The effects of heat acclimation on the expression of HaFT-1 and the role of this gene in cross\|stress response were analyzed through qRT-PCR, physiological indicator measurements, and cell staining methods. The results showed that after heat acclimation, the relative expression of the HaFT-1 gene in H. ammodendron roots under subsequent drought stress (3.81-fold) was significantly higher than under single drought treatment (2.92-fold). In A. thaliana, the seedling survival rate of HaFT-1 overexpressing lines under drought stress after heat acclimation (87.0%) was higher than under single drought treatment (81.7%), and both were higher than that of the wild type (approximately 76.7%). Additionally, leaf cell mortality in transgenic lines under subsequent drought stress (41.7%) was lower than under single drought stress (49.3%) and also lower than that of the wild type (47.7%). Physiological assays showed that transgenic lines exhibited higher antioxidant enzyme activities after stress, with the catalase (CAT) activity significantly increased under drought treatment after heat acclimation compared to the wild type (P<0.05). Gene expression analysis revealed that, under drought stress following heat acclimation, the expression levels of genes related to photosynthesis, proline synthesis, and stress response in transgenic lines were upregulated by 2- to 200-fold compared to the wild type. These results indicate that the HaFT-1 protein positively regulates plant tolerance to heat and drought cross\|stress by enhancing antioxidant defense, maintaining reactive oxygen species homeostasis, and modulating multiple stress response pathways. |
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