| Identification of SPX gene family in foxtail millet and analysis of its expression pattern in response to low phosphorus stress |
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| DOI：10.7606/j.issn.1000-7601.2026.02.17 |
| Key Words: Setaria italica L. SPX gene family low phosphorus stress expression analysis |
| Author Name | Affiliation | | GUO Haojie | Henan Dryland Crop Germplasm Resources Utilization Engineering Research Center, College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471000, China | | WANG Cheng | Henan Dryland Crop Germplasm Resources Utilization Engineering Research Center, College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471000, China | | YANG Furong | Henan Dryland Crop Germplasm Resources Utilization Engineering Research Center, College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471000, China | | DU Bing | Henan Dryland Crop Germplasm Resources Utilization Engineering Research Center, College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471000, China | | MENG Chaomin | Henan Dryland Crop Germplasm Resources Utilization Engineering Research Center, College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471000, China |
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| Abstract: |
| The whole\|genome identification and analysis of the SPX gene family in foxtail millet were carried out by bioinformatics methods. Combined with quantitative real\|time PCR (RT-qPCR) technology, the expression patterns of SPX gene family members in different tissues (roots, stems, leaves, and spikes) of foxtail millet and under low phosphorus stress were systematically analyzed to provide a theoretical basis for functional study and genetic improvement of the SPX gene family in foxtail millet. The results showed that there were 15 SPX members (SiSPX1-SiSPX15) in the SiSPX gene family of foxtail millet, which were unevenly distributed on 6 chromosomes, and there was gene fragment replication. Physical and chemical property analysis showed that the length of the amino acid sequence was between 252 and 849 aa, the molecular weight was between 28.22 and 97.22 KDa, and the isoelectric point was between 5.24 and 9.84. Phylogenetic analysis showed that the millet SPX gene family could be divided into four subfamilies (SPX, SPX-EXS, SPX-MFS, and SPX-RING). The members of the same subfamily had similar gene structures and conserved motifs. The analysis of promoter cis\|acting elements showed that promoter sequences of all members of the SPX gene family contain cis\|elements related to plant growth and develop\|ment, hormone response, and abiotic stress response. The results of RT-qPCR showed that the expression of the SiSPX gene had obvious tissue specificity, and its members were expressed in different tissues. Among them, SiSPX2/3/4/9/14/15 were highly expressed in roots, SiSPX5/7/8/10/13 were highly expressed in stems, and SiSPX1/6/11 were highly expressed in leaves. The analysis results under low phosphorus stress showed that the expression of 13 genes responded to low phosphorus stress and had different expression patterns at different treatment stages. SiSPX9 showed the highest expression level at 12 hours, SiSPX6 had the highest expression level at 72 hours, and SiSPX10 had the highest expression level at 4 hours. The expression of the above genes all showed highly significant differences compared to normal phosphorus\|supply treatment. |
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