| Genetic diversity and biological characteristics of endophytic bacteria isolated from root nodules of Astragalus mongholicus Bunge in eastern Inner Mongolia |
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| DOI：10.7606/j.issn.1000-7601.2026.02.23 |
| Key Words: Astragalus mongholicus Bunge endophytic bacteria in nodules genetic diversity biological characteristics |
| Author Name | Affiliation | | LI Hua | College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China | | YUAN Xiaoxia | College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China | | GAO Mengzhe | College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China | | YU Xiumin | College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China | | HUANG Hangbin | College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China | | JI Zhaojun | College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China
Key Laboratory of Mongolian Medicine Research and Development Engineering, Ministry of Education, Tongliao, Inner Mongolia 028000, China |
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| Abstract: |
| To explore the distribution type and genetic diversity of non\|symbiotic bacteria in the nodules of Astragalus mongholicus Bunge, the soil in eastern Inner Mongolia was collected to plant A. mongholicus, and the endophytic bacteria in root nodules were isolated and purified under aseptic conditions. The phylogenetic tree was constructed based on the 16S rDNA gene sequence, and the gene polymorphisms and biological characteristics of the isolates were analyzed. The results showed that 12 isolates were isolated and purified from the nodules of A. mongholicus, which belonged to Rhizobium (4 strains), Pseudomonas (6 strains), and Mesorhizobium (2 strains). Among them, R. mongolense and P. ficuserectae were the dominant species. The pairwise genetic distance (Dxy) between Pseudomonas and Rhizobium, and Mesorhizobium were 0.19110 and 0.17593, respectivley. The gene exchange index (Nm) was far less than 1.0, and no gene exchange occurred between them. The OD600 values of Pseudomonas in 3.0% NaCl, weak subacidity (pH=5), and strong alkali (pH=9) environments were all higher than 1.0, exhibiting strong salt, acid, and alkaline tolerance. Therefore, they were the excellent strains exploited for cultivation of Astragalus mongholicus Bunge inoculated by combined microbial agents of symbiotic and growth\|promoting bacteria in eastern Inner Mongolia with poor sandy soil. |
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