| In this study, 15 actinomycetes were isolated using three different media (M1～M3) from a salt pond of Qingdao. Bioinformatics analysis of the 16S rRNA sequences from the isolates revealed they belonged to three genera (Streptomyces, Nocardiopsis and a novel genus), with Streptomyces being the dominant genus. The strain CXB832 were 95.4 % and 94.9 % homologous to the two standard strains of bacteria, and therefore was candidates for a new genus on the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness. The type of medium and temperature showed significant effects on the number and diversity of actinomycetes recovered. Starch casein medium was the most effective media to isolate actinomycetes species at 37℃. 12, 3, 2 and 1 of 15 isolates produces amylase, lipase, caseinase and cellulose, respectively. |