| Regeneration pathways of Lycium ruthenicum Murr in vitro |
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| DOI：10.7606/j.issn.1000-7601.2018.05.09 |
| Key Words: Lycium ruthenicum Murr explant callus clump buds regenerated plants |
| Author Name | Affiliation | | CAO Jun-mai | College of Biological Sciences and Engineering, Beifang University of Nationalities, Yinchuan, Ningxia 750021, China | | MA Hai-jun | College of Biological Sciences and Engineering, Beifang University of Nationalities, Yinchuan, Ningxia 750021, China | | TAN Ya-ping | College of Biological Sciences and Engineering, Beifang University of Nationalities, Yinchuan, Ningxia 750021, China |
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| Abstract: |
| Effects of hormones on the regeneration pathway of callus-induced plantlets, clump shoot-induced plantlets and leaf\\induced plantlets were investigated by explants, leaves and stem segments of Lycium ruthenicum Murr and MS basic medium. The results showed that the appropriate medium for callus induction from stem segments and leaves was MS+2, 4-D 0.3 mg·L-1 and MS+2, 4-D 0.4 mg·L-1, respectively and both of the induction rate were 100%. The appropriate medium for differentiation of stem segments and calli into clump shoot was MS+6-BA 0.2 mg·L-1+KT 0.1 mg·L-1 and MS+6-BA 0.5 mg·L-1, respectively, with multiplication coefficient of 32.3 times and 47.1 times, respectively. However, vitrification occurred in clump shoot differentiated from calli. The appropriate medium for inducing leaves to differentiate into plants was MS+NAA 0.01 mg·L-1, with induction rate of regenerated plants of 33.3%. The regeneration ability of Lycium ruthenicum Murr was strong, and regenerated plants could be formed through above pathways. The optimal in vitro propagation pathway was stem segments inducing clump shoot to form regenerated plants. |
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